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| Status |
Public on Feb 05, 2019 |
| Title |
Placenta-specific DMRs maintain methylation across gestation. |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
Methylation profiling by array
|
| Summary |
One possible mechanism leading to the apparent polymorphic placenta-specific DMRs would be the failure to maintain allelic methylation during gestation. For a temporal comparison, we performed methylation profiling on first trimester chorionic villus sampling (CVS) and compared it with corresponding samples at term. This revealed that DNA methylation level at placenta-specific DMRs is highly stable between the two points. In addition, to ensure that the methylation profiles were uniform across the placental plate, we determined the placenta-specific DMR profiles from multiple biopsies from the same term placentas. Biopsies collected from the opposite sides of the cord insertion site also showed high correlations, suggesting that methylation does not vary greatly between sampling sites. Finally, we compared placenta samples from dizygotic twins and triplets. As in the other cases, this revealed that the correlations between samples of the same gestations (sharing the same in utero environment and maternal exposures) were also higher than between unrelated samples.
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| Overall design |
Bisulphite converted DNA from 12 human placenta samples (two pairs of first trimester CVS and samples at term, two pairs of opposite sides biopsies from the same term placentas, one pair of dyzigotic twin term placentas and one trio of triplet placenta samples) were hybridised to the Illumina Infinium HumanMethylationEPIC and HumanMethylation450 BeadChips.
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| Contributor(s) |
Hernandez JR, Monk D |
| Citation(s) |
30808399, 31357948, 31847873 |
| Submission date |
Oct 10, 2018 |
| Last update date |
Dec 31, 2019 |
| Contact name |
Jose Ramon Hernandez |
| Organization name |
Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)
|
| Department |
Cancer Epigenetics and Biology Program (PEBC)
|
| Lab |
Imprinting and Cancer group
|
| Street address |
Av. Gran Via de l'Hospitalet 199-203
|
| City |
L'Hospitalet de Llobregat, Barcelona |
| ZIP/Postal code |
08907 |
| Country |
Spain |
| |
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| Platforms (2) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
| GPL21145 |
Infinium MethylationEPIC |
|
| Samples (12)
|
| GSM3425289 |
bisulphite converted DNA from control placenta BC162905231 |
| GSM3425290 |
bisulphite converted DNA from control placenta PL4853477_new |
| GSM3425291 |
bisulphite converted DNA from control placenta BCA16172 |
| GSM3425292 |
bisulphite converted DNA from control placenta PL4881886 |
| GSM3425293 |
bisulphite converted DNA from control placenta PL58 |
| GSM3425294 |
bisulphite converted DNA from control placenta PL59 |
| GSM3425295 |
bisulphite converted DNA from control placenta PL60 |
| GSM3425296 |
bisulphite converted DNA from control placenta PL179 |
| GSM3425297 |
bisulphite converted DNA from control placenta PL180 |
| GSM3425298 |
bisulphite converted DNA from control placenta PL55_b |
| GSM3425299 |
bisulphite converted DNA from control placenta PL55 |
| GSM3425300 |
bisulphite converted DNA from control placenta PL58_b |
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| Relations |
| BioProject |
PRJNA495474 |
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