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| Status |
Public on Jul 07, 2009 |
| Title |
Expression data in polysomal and total RNA from perinatal rat lung |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
During the peri-partum period, the lung must respond to many factors with potential to impact protein synthesis via regulation of translation initiation. Microarray analysis of polysomal versus total RNA from fetal day (FD) 19, FD22 and postnatal day 1 (P1) rat lungs was used to identify genes whose association with large polysomes changed either pre- or postnatally.
Keywords: development
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| Overall design |
Using three independent litters at each developmental time point, we prepared lysates from FD19, FD22 and P1 (5 h after birth) rat lungs (total 9 lysates). An aliquot of each lysate was used to prepare total RNA. Remaining lysate was subjected to sucrose density gradient centrifugation to isolate large polysomes ( n>7 ribosomes/mRNA) from which we isolated polysomal RNA. Matched total and polysomal RNAs were hybridized to Affymetrix expression arrays (total 18 chips).
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| Contributor(s) |
Otulakowski G, Duan W, O'Brodovich H |
| Citation(s) |
18952566 |
| Submission date |
Jul 17, 2008 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Gail Otulakowski |
| Organization name |
Hospital for Sick Children Research Institute
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| Department |
Physiology & Experimental Medicine
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| Street address |
555 University Ave
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| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5G 1X8 |
| Country |
Canada |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (18)
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| GSM306043 |
FD19 lung, total RNA, biological replicate 1 |
| GSM306044 |
FD19 lung, total RNA, biological replicate 2 |
| GSM306045 |
FD19 lung, total RNA, biological replicate 3 |
| GSM306046 |
FD19 lung, polysomal RNA, biological replicate 1 |
| GSM306047 |
FD19 lung, polysomal RNA, biological replicate 2 |
| GSM306048 |
FD19 lung, polysomal RNA, biological replicate 3 |
| GSM306049 |
FD22 lung, total RNA, biological replicate 1 |
| GSM306050 |
FD22 lung, total RNA, biological replicate 2 |
| GSM306051 |
FD22 lung, total RNA, biological replicate 3 |
| GSM306052 |
FD22 lung, polysomal RNA, biological replicate 1 |
| GSM306053 |
FD22 lung, polysomal RNA, biological replicate 2 |
| GSM306054 |
FD22 lung, polysomal RNA, biological replicate 3 |
| GSM306055 |
P1 lung, total RNA, biological replicate 1 |
| GSM306056 |
P1 lung, total RNA, biological replicate 2 |
| GSM306057 |
P1 lung, total RNA, biological replicate 3 |
| GSM306058 |
P1 lung, polysomal RNA, biological replicate 1 |
| GSM306059 |
P1 lung, polysomal RNA, biological replicate 2 |
| GSM306060 |
P1 lung, polysomal RNA, biological replicate 3 |
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| Relations |
| BioProject |
PRJNA113769 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE12153_RAW.tar |
51.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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