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| Status |
Public on Oct 24, 2018 |
| Title |
Essential role of Arid1a in intestinal stem cell maintenance and homeostasis through Sox9 regulation (ChIP-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Arid1a, a subunit of the SWI/SNF chromatin remodeling complex, have been reported in multiple human cancers, however, its functional role in intestinal homeostasis remains unclear. To investigate the role of Arid1a in murine intestine, we have employed ChIP experiments in intestinal spheroid cells generated from crypt cells of wild type mice. ChIP assay revealed that Arid1a binds directly to the Sox9 promoter to support its expression.
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| Overall design |
We used intestinal spheroid cells generated from crypt cells of wild type mice.
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| Contributor(s) |
Hiramatsu Y, Fukuda A |
| Citation(s) |
30635419 |
| Submission date |
Oct 23, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Yukiko Hiramatsu |
| E-mail(s) |
hirayuki@kuhp.kyoto-u.ac.jp
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| Organization name |
Kyoto University Graduate School of Medicine
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| Department |
Department of Gastroenterology and Hepatology
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| Street address |
54 Shogoin-Kawahara-cho, Sakyo-ku
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| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA498053 |
| SRA |
SRP166448 |
