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Series GSE12170 Query DataSets for GSE12170
Status Public on Sep 16, 2008
Title Global Analysis of the Meiotic Crossover Landscape
Organism Saccharomyces cerevisiae
Experiment type Genome variation profiling by array
Summary Using microarrays to genotype the parental origin of progeny resulting from a cross between S96 and YJM789 yeast strains, we mapped the distribution of crossovers that occurred during meiosis. Knowledge of the crossover distribution allowed us to assess changes in crossover control in wild type and mutant strains.
The S96 strain is a S288 derivative and thus its DNA sequence has high homology to the oligo sequences used to create the S98 Affymetrix Gene chip. The YJM789 strain is ~ 0.6% divergent from S288.

Keywords: wild type and mutant analysis
 
Overall design Strains were allowed to undergo sporulation (meiosis) and progeny (spores) from 4-spore viable tetrads were grown and genomic DNA was extracted and hybridzed to S98 Affymetrix Gene chips. Segregation patterns were analyzed for the location of crossovers which are represented by reciprocal exchange of DNA.
 
Contributor(s) Chen S, Fung J
Citation(s) 18691940
Submission date Jul 18, 2008
Last update date Jul 01, 2016
Contact name Jennifer Fung
E-mail(s) jfung@biochem.ucsf.edu
Phone 415 514-4309
Fax 415 514-4145
Organization name UCSF
Department Biochemistry and Biophysics
Lab Fung
Street address S316 Genentech Hall, MC2200 600 16th St.
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platforms (1)
GPL90 [YG_S98] Affymetrix Yeast Genome S98 Array
Samples (140)
GSM306312 wild type tetrad 5, spore A, B, C, D
GSM306313 wild type tetrad 6, spore A, B, C, D
GSM306314 wild type tetrad 10, spore A, B, C, D
Relations
BioProject PRJNA113459

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE12170_RAW.tar 1.2 Gb (http)(custom) TAR (of CEL, TXT)
Processed data provided as supplementary file

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