 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Oct 23, 2021 |
| Title |
Omeprazole regulates miRNA, mRNA and lncRNA networks revealed by RNA deep-sequencing in Barrett's Esophagus |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Recent clinical studies indicated Proton-pump inhibitors (PPIs) but not H2 Receptor Antagonists were associated with a decreased risk of esophageal adenocarcinoma. We’d like to clarify whether PPIs interfere with Barrett’s esophagus(BE) pathogenesis during BE treatment and explore the novel roles of omeprazole beyond acid suppression. RNA deep-sequencing was conducted in BE organoids exposed to periodic bile acids(400µM, pH 5.5) stimulation with or without omeprazole（40µM）treatment. A total of 129 long non-coding RNAs, 20 microRNAs, and 285 mRNAs were significantly regulated. Then, bioinformatics tools and databases were employed to explore the potential functions and relationships of these RNAs. Our data showed that the most significantly involved pathways modulated by omeprazole were Phenylalanine metabolism and Glycosaminoglycan biosynthesis - keratan sulfate. In addition, the miRNA-mRNA-lncRNA network regulated by omeprazole was constructed. We have demonstrated some novel acid-independent mechanisms of omeprazole that might yield valuable insight into clinical management of BE, irrespective of acid reflux symptoms.
|
| |
|
| Overall design |
RNA deep-sequencing was conducted in BE organoids exposed to periodic bile acids (400µM, pH 5.5) stimulation with or without omeprazole (40µM) treatment.
|
| |
|
| Contributor(s) |
Xiong H, Huang J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 25, 2018 |
| Last update date |
Oct 23, 2021 |
| Contact name |
Hua Xiong |
| E-mail(s) |
huaxiong1979@163.com
|
| Phone |
18701837151
|
| Organization name |
Division of Gastroenterology and Hepatology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University
|
| Street address |
Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Cancer Institute,Shanghai, Institute of Digestive Disease, 145 Middle Shandong Road, Shanghai 200001, China
|
| City |
Shanghai |
| State/province |
上海市 |
| ZIP/Postal code |
200001 |
| Country |
China |
| |
|
| Platforms (1) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA498429 |
| SRA |
SRP166865 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE121788_lncRNA.txt.gz |
4.5 Mb |
(ftp)(http) |
TXT |
| GSE121788_mRNA.txt.gz |
2.7 Mb |
(ftp)(http) |
TXT |
| GSE121788_microRNA.txt.gz |
22.0 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...