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Series GSE12189 Query DataSets for GSE12189
Status Public on Jul 10, 2009
Title FACS-Assisted Microarray Profiling Implicates Novel Genes and Pathways in Zebrafish Gastrointestinal Tract Development
Organism Danio rerio
Experiment type Expression profiling by array
Summary Zebrafish (Danio rerio) gutGFP transgenic embryos [Tg(XlEef1a1:GFP)s854] were collected at 4 time points: 2 days post fertilization (dpf), 3, dpf, 4 dpf, 6 dpf. Embryos were dissociated into single cells and sorted by FACS based on GFP expression.
RNA was extracted from the different cell populations (Stratagene), amplified (NuGEN Ovation), and hybridized to Affymetrix Zebrafish GeneChips.

Keywords: Time course.
 
Overall design Series of 24 samples: 4 time points, 3 replicates, 2 samples per replicate (GFP+ and GFP-).
 
Contributor(s) Stuckenholz C, Lu L, Thakur P, Kaminski N, Bahary N
Citation(s) 19563808
Submission date Jul 21, 2008
Last update date Jan 25, 2018
Contact name Carsten Stuckenholz
E-mail(s) cstucken@pitt.edu
Phone (412) 648-3159
Fax (412) 383-5918
Organization name University of Pittsburgh
Department Department of Medicine, Division of Hematology and Oncology
Lab Bahary
Street address 3501 Fifth Avenue, Room 5065.5B
City Pittsburgh
State/province PA
ZIP/Postal code 15260
Country USA
 
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (24)
GSM306831 s854 2dpf GFP+ Rep1
GSM306832 s854 2dpf GFP- Rep1
GSM306833 s854 2dpf GFP+ Rep2
Relations
BioProject PRJNA113405

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE12189_RAW.tar 49.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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