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| Status |
Public on Nov 27, 2018 |
| Title |
polyA RNA Sequencing Analysis of HTR-8/SVneo cells after lnc-SLC4A1-1 overexpression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To further evaluate the functional role of lnc- SLC4A1-1, we overexpressed lnc-SLC4A1-1 in HTR-8/SVneo cells and isolated RNA for RNA-seq. RNA-seq was done using TruSeq Stranded mRNA Library Preparation. Briefly, intact RNA was fragmented, end repaired, adapter ligation and PCR amplified following illumina protocol. Libraries were sequencing by illumins Hiseq 3000. After quality control, sequence data were processed with STAR to generate read alignments with hg19. Raw read counts for annotated genes were obtained with featureCounts with default settings, normalized and analyzed using DEGseq.
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| Overall design |
Intact RNA isolated from 6 human HTR-8/SVneo cells was fragmented, end repaired, adapter ligation and PCR amplified following illumina protocol. Libraries were sequencing by illumins Hiseq 2000.
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| Contributor(s) |
Huang Z, Lu C |
| Citation(s) |
30448228 |
| Submission date |
Oct 29, 2018 |
| Last update date |
Dec 23, 2018 |
| Contact name |
Zhenyao Huang |
| E-mail(s) |
huangzhenyao@gmail.com
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| Organization name |
Nanjing Medical University
|
| Street address |
Dongshan
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| City |
Nanjing |
| ZIP/Postal code |
211166 |
| Country |
China |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA499120 |
| SRA |
SRP167170 |
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