|
| Status |
Public on Nov 01, 2018 |
| Title |
Gene expression profiles of isogenic single-cell derived clones of BRAF-mutated SK-MEL-5 melanoma cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We recently reported that single-cell derived isogenic subclones of SKMEL5 cells have differential initial sensitivity to BRAF-inhibitors. In order to probe differences among these subclones, we selected three subclones with unique drug responses: progressing (SK-MEL-5 SC10), stationary (SK-MEL-5 SC07), and regressing (SK-MEL-5 SC01) and performed RNASeq. This study examines differentially expressed genes (DEGs) among the subclones to identify the molecular basis for initial differences in drug sensitivity.
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| |
|
| Overall design |
Transcriptomics analysis between single-cell derived isogenic subclones of BRAF-mutated melanoma cell line, SK-MEL-5
|
| |
|
| Contributor(s) |
Paudel B, Quaranta V |
| Citation(s) |
29590606, 33060170 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 CA186193 |
Quantitative Multiscale Imaging to Optimize Cancer Treatment Strategies |
Vanderbilt University Medical Center |
Vito Quaranta |
| U01 CA174706 |
Image Driven Multi-Scale Modeling to Predict Treatment Response in Breast Cancer |
UNIVERSITY OF TEXAS AUSTIN |
Vito Quaranta |
|
| Submission date |
Oct 31, 2018 |
| Last update date |
Sep 29, 2021 |
| Contact name |
B Bishal Paudel |
| E-mail(s) |
buddhi.b.paudel@vanderbilt.edu
|
| Organization name |
Vanderbilt University
|
| Department |
Biochemistry
|
| Street address |
2220 Pierce Ave PRB447
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA503257 |
| SRA |
SRP167389 |
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