NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE122182 Query DataSets for GSE122182
Status Public on Nov 06, 2018
Title Expression data of miRNA profile from colorectal cancer and the adjacent normal tissues infected with F. nucleatum
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Expression data of miRNA profile from colorectal cancer and the adjacent normal tissues infected with F. nucleatum. Colorectal cancer(CRC) is among the most common cancer types in the world and one of the most lethal cancers. Fusobacterium nucleatum (F. nucleatum) plays an etiologic role in the development of colorectal cancer, but specific tumor molecules involved in the progression of CRC induced by F. nucleatum is not clear. This study investigated some miRNAs and the genes involved in the progression of F. nucleatum-induced colorectal cancer by Affymetrix miRNA microarray technology.
We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated and down-regulated genes between Colorectal cancer and the adjacent normal tissues (CONTROL) infected without F. nucleatum . The miRNA array was performed using the affymetrix GeneChip® miRNA 3.0 Array (Affymetrix, Santa Clara, California, United States). The chip was processed using a commercial Affymetrix array service (GeneTech Biotechnology Limited Company, Shanghai, China). The affymetrix GeneChip® miRNA 3.0 Array contains 2,999 probe sets unique to human, mouse and rat pre-miRNA hairpin sequences, 2,216 human snoRNA and scaRNA probe sets and covers 153 organisms (19,724 probe sets). Raw data sets were extracted from all Cel files (raw intensity file) after scanning of slides. These raw data sets were separately analyzed using Expression Console and GeneSpring GX12.5 software followed by differential miRNA expression, fold change & cluster analysis.
 
Overall design Affymetrix GeneChip® miRNA 3.0 Array were used to analysis 25 specimens (15 F. nucleatum-induced colorectal cancer, 10 F. nucleatum-induced control).
 
Contributor(s) Tang B
Citation(s) 30951563, 37216106
Submission date Nov 05, 2018
Last update date Aug 08, 2023
Contact name bin tang
E-mail(s) tangbin811012@163.com
Phone 15281936788
Organization name Southwest Hospital &College of Medical Laboratory Science; Third Military Medical University
Department Department of Clinical Microbiology and Immunology
Street address Southwest Hospital &College of Medical Laboratory Science; Third Military Medical University
City chongqing
State/province chongqing
ZIP/Postal code 400038
Country China
 
Platforms (1)
GPL16384 [miRNA-3] Affymetrix Multispecies miRNA-3 Array
Samples (25)
GSM3457322 Early stage of colorectal cancer tissues without F. nucleatum infection,rep1
GSM3457323 Early stage of colorectal cancer tissues without F. nucleatum infection,rep2
GSM3457324 Early stage of colorectal cancer tissues without F. nucleatum infection,rep3
Relations
BioProject PRJNA503886

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE122182_RAW.tar 40.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap