|
| Status |
Public on Nov 06, 2018 |
| Title |
Analyses of long non-coding RNAs in human retinal endothelial cells following glucose treatment |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
Non-coding RNA profiling by array
|
| Summary |
The differential expression of lncRNAs in human retinal endothelial cells (HRECs) was investigated with a commericially available microarray (ArrayStar Human LncRNA Microarray V3.0; ArrayStar Inc., Rockville, Maryland USA). Approximately 28,035 lncRNAs and 21,407 coding transcripts were detected using this microarray. In addition to the array findings, lncRNAs were further constructured using transcriptome databases (including RefSeq, Gencode, UCSC). Sequences were selected using proprietary strategies and each transcript is represented by a specific exon or splice junction probe, which can accurately identify an individual transcript. Nevertheless, both postive and negative probes for housekeeping genes were additionally used as controls.
|
| |
|
| Overall design |
In the present study, two control groups (normal glucose conditions, 5 mM/L; and high glucose, 25 mM/L) and two treatment groups (high glucose conditions, 25 mM/L) were established using HRECs.
|
| |
|
| Contributor(s) |
Chakrabarti SS, Chen SS, Biswas SS |
| Citation(s) |
30612136, 32655995 |
| Submission date |
Nov 05, 2018 |
| Last update date |
Aug 04, 2020 |
| Contact name |
Saumik Biswas |
| E-mail(s) |
sbiswas7@uwo.ca
|
| Phone |
519-661-2111
|
| Organization name |
University of Western Ontario
|
| Department |
Pathology and Laboratory Medicine
|
| Lab |
Chakrabarti Lab (DSB 4033)
|
| Street address |
1151 Richmond Street
|
| City |
London |
| State/province |
Ontario |
| ZIP/Postal code |
N6A 5C1 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL16956 |
Agilent-045997 Arraystar human lncRNA microarray V3 (Probe Name Version) |
|
| Samples (4)
|
| GSM3457447 |
HRECs-48hr-normal glucose treatment-rep1 |
| GSM3457448 |
HRECs-48hr-normal glucose treatment-rep2 |
| GSM3457449 |
HRECs-48hr-high glucose treatment-rep1 |
|
| Relations |
| BioProject |
PRJNA503895 |
Less...
More...