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| Status |
Public on Aug 01, 2008 |
| Title |
Iodide Inhibits Vascular Endothelial Growth Factor-A Expression in Cultured Human Thyroid Follicles |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
A Microarray Search for Effects of Thyrotropin and Iodide on Angiogenesis Factors
Objective: Excess iodide has been administered to hyperthyroid patients before thyroid surgery to reduce intraoperative bleeding and oozing. The purpose of this study was to elucidate the mechanism by which iodide reduces blood flow in the hypervascular thyroid gland. Design: Human thyroid follicles were cultured in the presence or absence of thyrotropin (TSH), or in medium containing various concentrations of iodide, and TSH- or iodide-regulated gene expression was analyzed by cDNA microarray. Main outcome: TSH stimulated the expression of thyroglobulin, peroxidase, sodium-iodide symporter, vascular endothelial growth factor (VEGF)-A, VEGF-B, and placental growth factor (PGF) but decreased that of VEGF-C by half. When thyroid follicles were cultured in high-iodide (10-5M) medium, TSH-induced expression of VEGF-A, VEGF-B and PGF was decreased, accompanied by a reduction of VEGF-A release into the medium. Furthermore, expression of putative angiogenesis inhibitors such as urokinase-type plasminogen activator (PLAU) was increased. These findings were confirmed by real-time polymerase chain reaction (PCR) and Northern blot hybridization. Conclusions: We have demonstrated for the first time that iodide at high concentration decreases the expression of the angiogenic factors VEGF-A, VEGF-B, and PGF, accompanied by an increase in the expression of possible anti-angiogenic factors such as PLAU. These proangiogenic and antiangiogenic factors may at least partly account for the iodide-induced decrease in thyroid blood flow.
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| Overall design |
One conditioned experiments:
control vs. bovine thyrotropin (bTSH) 30 microUnit/ml
iodide 1x10e-8M vs. iodide 2x10e-5M
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| Contributor(s) |
Sato K, Yamada E |
| Citation(s) |
16839256 |
| Submission date |
Jul 25, 2008 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Kanji Sato |
| E-mail(s) |
satokan@attglobal.net
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| Phone |
81-3-3353-8111
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| Fax |
81-3-3354-3706
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| Organization name |
Tokyo Women's Medical University
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| Department |
Institute of Clinical Endocrinology
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| Lab |
Department of Medicine
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| Street address |
Kawada-cho 8-1
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| City |
Shinjuku-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
162-8666 |
| Country |
Japan |
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| Platforms (1) |
| GPL1708 |
Agilent-012391 Whole Human Genome Oligo Microarray G4112A (Feature Number version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA113709 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE12244_RAW.tar |
26.5 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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