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Series GSE122815 Query DataSets for GSE122815
Status Public on Jun 10, 2019
Title Expression data from mouse brain in response to intracerebral LPS challenge
Organism Mus musculus
Experiment type Expression profiling by array
Summary A model of self-limiting acute brain inflammation induced by intracerebral injection of LPS was validated in the mouse tailored to enable study of inflammation resolution.
Transcriptome-wide changes in gene expression were assessed by microarray at induction and resolution phases of the inflammatory response
 
Overall design C57Bl/6J mice received intrastriatal injection of LPS or PBS and brain samples were dissected from the injected hemisphere after transcardiac saline perfusion for mRNA and miRNA extraction. Samples were collected 1 d after LPS (induction phase, n=9), 4 d after LPS (resolution phase, n=9) or 4 d after PBS (n=9) treatments. For each treatment group, samples were pooled to form three biological replicates for microarray i.e. each replicate comprised a pooled RNA sample derived from 3 different mice (with equivalent quantity of RNA from each constituent sample).
 
Contributor(s) McColl BW, Davies CL
Citation(s) 31156629
Submission date Nov 21, 2018
Last update date Jun 10, 2019
Contact name Barry W McColl
E-mail(s) barry.mccoll@ed.ac.uk
Organization name University of Edinburgh
Department Centre for Discovery Brain Sciences
Street address 49 Little France Crescent
City Edinburgh
ZIP/Postal code EH16 4SB
Country United Kingdom
 
Platforms (1)
GPL11180 [HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate
Samples (9)
GSM3485901 ipsilateral brain_LPS+1d_biological rep1
GSM3485902 ipsilateral brain_LPS+1d_biological rep2
GSM3485903 ipsilateral brain_LPS+1d_biological rep3
Relations
BioProject PRJNA506425

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE122815_RAW.tar 20.6 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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