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Status |
Public on Jan 01, 2009 |
Title |
Interleukin-1-induced gene expression in mouse dermal fibroblasts |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Interleukin-1 (IL-1) is produced from immune-activated cells upon infection and tissue injury, and stimulates a variety of cells expressing its receptor. IL-1 receptor activation results in intracellular signaling cascades that culminate in the activation of transcription factors such as NF-kappaB, AP-1, and C/EBPbeta. To identify genes whose expression is induced in response to IL-1, we performed DNA microarray analysis of IL-1-treated murine dermal fibroblasts (DFs). The gene expression patterns in control, untreated DFs and in DFs treated with IL-1 were compared.
Keywords: Time course
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Overall design |
Total RNAs from untreated DFs (0 h) vs. IL-1-treated DFs (1 h, 2 h, or 4 h after IL-1 treatment) were isolated and analyzed using an Agilent DNA microarray platform. Hybridization was performed in duplicate for dye swapping.
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Contributor(s) |
Park JM, Aoki H |
Citation missing |
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Submission date |
Jul 29, 2008 |
Last update date |
Dec 06, 2012 |
Contact name |
Jin Mo Park |
E-mail(s) |
jmpark@cbrc2.mgh.harvard.edu
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Phone |
617-643-2328
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Organization name |
Massachusetts General Hospital
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Department |
Cutaneous Biology Research Center
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Street address |
149 Thirteenth Street
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City |
Charlestown |
State/province |
MA |
ZIP/Postal code |
02129 |
Country |
USA |
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Platforms (1) |
GPL2872 |
Agilent-012694 Whole Mouse Genome G4122A (Feature Number version) |
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Samples (6)
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Relations |
BioProject |
PRJNA113569 |