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Series GSE12285 Query DataSets for GSE12285
Status Public on Jan 01, 2009
Title Interleukin-1-induced gene expression in mouse dermal fibroblasts
Organism Mus musculus
Experiment type Expression profiling by array
Summary Interleukin-1 (IL-1) is produced from immune-activated cells upon infection and tissue injury, and stimulates a variety of cells expressing its receptor. IL-1 receptor activation results in intracellular signaling cascades that culminate in the activation of transcription factors such as NF-kappaB, AP-1, and C/EBPbeta. To identify genes whose expression is induced in response to IL-1, we performed DNA microarray analysis of IL-1-treated murine dermal fibroblasts (DFs). The gene expression patterns in control, untreated DFs and in DFs treated with IL-1 were compared.

Keywords: Time course
 
Overall design Total RNAs from untreated DFs (0 h) vs. IL-1-treated DFs (1 h, 2 h, or 4 h after IL-1 treatment) were isolated and analyzed using an Agilent DNA microarray platform. Hybridization was performed in duplicate for dye swapping.
 
Contributor(s) Park JM, Aoki H
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Submission date Jul 29, 2008
Last update date Dec 06, 2012
Contact name Jin Mo Park
E-mail(s) jmpark@cbrc2.mgh.harvard.edu
Phone 617-643-2328
Organization name Massachusetts General Hospital
Department Cutaneous Biology Research Center
Street address 149 Thirteenth Street
City Charlestown
State/province MA
ZIP/Postal code 02129
Country USA
 
Platforms (1)
GPL2872 Agilent-012694 Whole Mouse Genome G4122A (Feature Number version)
Samples (6)
GSM308550 DF 1 h (Cy5) vs. 0 h (Cy3)
GSM308551 DF 1 h (Cy3) vs. 0 h (Cy5)
GSM308552 DF 2 h (Cy5) vs. 0 h (Cy3)
Relations
BioProject PRJNA113569

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE12285_RAW.tar 76.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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