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Series GSE12299 Query DataSets for GSE12299
Status Public on Oct 20, 2008
Title Gene expression in response to depletion of specific human histone H1 isoforms.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary At least six histone H1 variants exist in mammalian somatic cells that bind to the linker DNA and stabilize the nucleosome particle contributing to higher order chromatin compaction. In addition, H1 seems to be involved in the active regulation of gene expression. It is not well known whether the different variants have specific roles or regulate specific promoters. We have explored this by inducible shRNA-mediated knock-down of each of the H1 variants in a human breast cancer cell line. A different subset of genes is altered in each H1 knock-down.

Keywords: cell type comparison; genetic modification; RNA interference
 
Overall design Stable breast cancer-derived cell lines expressing an shRNA against one of each of the histone H1 isoforms in response to doxycycline (Dox) were grown for six days in the presence or absence of Dox, in duplicate, and RNA extracted for microarray hybridization. Cell lines used: inducible shRNA against H1.0, H1.2, H1.3, H1.4, H1.5, and control/empty shRNA-expression vector.
 
Contributor(s) Jordan A, Sancho M, Sumoy L
Citation(s) 18927631, 28977426
Submission date Jul 30, 2008
Last update date Jan 12, 2018
Contact name Lauro Sumoy
E-mail(s) lsumoy@igtp.cat
Organization name IGTP
Department High Content Genomics and Bioinformatics
Street address Ctra. Can Ruti, Camí de les escoles s/n
City Badalona
State/province Barcelona
ZIP/Postal code 08916
Country Spain
 
Platforms (1)
GPL6104 Illumina humanRef-8 v2.0 expression beadchip
Samples (24)
GSM309316 control -dox repl.1
GSM309317 control -dox repl.2
GSM309318 control +dox repl.1
Relations
BioProject PRJNA113597

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE12299_RAW.tar 3.4 Mb (http)(custom) TAR
GSE12299_raw_data.txt 31.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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