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| Status |
Public on Aug 08, 2019 |
| Title |
TET-catalyzed 5-carboxylcytosine promotes CTCF binding to suboptimal sequences genome-wide |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
The mechanisms supporting dynamic regulation of CTCF binding sites remain poorly understood. Here we describe the TET-catalyzed 5-methylcytosine derivative, 5-carboxylcytosine (5caC) as a factor driving new CTCF binding within genomic DNA. Through a combination of in vivo and in vitro approaches, we reveal that 5caC generally strengthens CTCF association with DNA and facilitates binding to suboptimal sequences. Dramatically, profiling of CTCF binding in a cellular model that accumulates genomic 5caC identified ∼13,000 new CTCF sites. The new sites were enriched for overlapping 5caC and were marked by an overall reduction in CTCF motif strength. As CTCF has multiple roles in gene expression, these findings have wide-reaching implications and point to induced 5caC as a potential mechanism to achieve differential CTCF binding in cells.
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| |
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| Overall design |
Characterization of CTCF binding in TDG KO condition compared to wild type, and examination of 5caC
|
| Web link |
https://0-doi-org.brum.beds.ac.uk/10.1016/j.isci.2019.07.041
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| |
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| Contributor(s) |
Nanan K, Sturgill D, Prigge MF, Thenoz M, Dillman AA, Mandler MD, Oberdoerffer S |
| Citation(s) |
31404833 |
| Submission date |
Nov 29, 2018 |
| Last update date |
Aug 27, 2019 |
| Contact name |
David Sturgill |
| E-mail(s) |
davidsturgill@mail.nih.gov
|
| Phone |
240-760-6725
|
| Organization name |
NIH
|
| Department |
NCI
|
| Lab |
LRBGE
|
| Street address |
Building 41, Rm B622
|
| City |
Bethesda |
| State/province |
MARYLAND |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
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| Platforms (2) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA507546 |
| SRA |
SRP171121 |
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