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Status |
Public on Dec 30, 2011 |
Title |
Gene expression analysis of TGF-beta signaling-disrupting gastric cancer cell line 2MLN |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Diffuse-type gastric carcinoma is a poor-prognostic cancer with high expression of transforming growth factor (TGF)-β and thick stromal fibrosis. However, detailed investigations on the roles of TGF-β signaling in diffuse-type gastric carcinoma have not been performed. We generated two diffuse-type gastric carcinoma cell lines, dominant-negative TGF-β type II receptor expressing cells (2MLN-dnTβRII) and GFP-expressing cells (2MLN-GFP). Cells were subcutaneously or orthotopically injected into nude mice. Although dnTβRII did not affect the growth of OCUM-2MLN in vitro, it accelerated the growth of subcutaneously or orthotopically transplanted tumors in vivo. By microarray analysis, we found gene expression of TSP-1, an angiogenic inhibitor, was down-regulated in dnTβRII tumors. This results suggested disruption of TGF-β signaling in diffuse-type gastric carcinoma cells leads to alteration of tumor microenvironment and acceleration of tumor growth.
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Overall design |
We generated two OCUM-2MLN-derived gastric carcinoma cell lines; 2MLN-dnTβRII, which expresses dominant negative form of TβRII, and control 2MLN-GFP, which expresses GFP. The transplanted tumors of these cell lines were subjected to gene expression analysis with Affymetrix U133 plus2 arrays.
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Contributor(s) |
Komuro A, Yashiro M, Iwata C, Morishita Y, Johansson E, Matsumoto Y, Watanabe A, Aburatani H, Kiyono K, Shirai Y, Suzuki HI, Hirakawa K, Kano MR, Miyazono K |
Citation(s) |
19351925 |
Submission date |
Aug 05, 2008 |
Last update date |
Mar 25, 2019 |
Contact name |
Mitsunobu R Kano |
E-mail(s) |
mikano-tky@umin.net
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Phone |
+81-3-5841-3356
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Fax |
+81-3-5841-3354
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Organization name |
The University of Tokyo
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Department |
Graduate School of Medicine
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Lab |
Department of Molecular Pathology
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Street address |
Hongo 7-3-1
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City |
Bunkyo-ku |
State/province |
Tokyo |
ZIP/Postal code |
113-0033 |
Country |
Japan |
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Platforms (1) |
GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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Samples (2) |
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Relations |
BioProject |
PRJNA113229 |
Supplementary file |
Size |
Download |
File type/resource |
GSE12336_RAW.tar |
15.2 Mb |
(http)(custom) |
TAR (of CEL) |
Processed data included within Sample table |
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