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Series GSE12336 Query DataSets for GSE12336
Status Public on Dec 30, 2011
Title Gene expression analysis of TGF-beta signaling-disrupting gastric cancer cell line 2MLN
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Diffuse-type gastric carcinoma is a poor-prognostic cancer with high expression of transforming growth factor (TGF)-β and thick stromal fibrosis. However, detailed investigations on the roles of TGF-β signaling in diffuse-type gastric carcinoma have not been performed. We generated two diffuse-type gastric carcinoma cell lines, dominant-negative TGF-β type II receptor expressing cells (2MLN-dnTβRII) and GFP-expressing cells (2MLN-GFP). Cells were subcutaneously or orthotopically injected into nude mice. Although dnTβRII did not affect the growth of OCUM-2MLN in vitro, it accelerated the growth of subcutaneously or orthotopically transplanted tumors in vivo. By microarray analysis, we found gene expression of TSP-1, an angiogenic inhibitor, was down-regulated in dnTβRII tumors. This results suggested disruption of TGF-β signaling in diffuse-type gastric carcinoma cells leads to alteration of tumor microenvironment and acceleration of tumor growth.
 
Overall design We generated two OCUM-2MLN-derived gastric carcinoma cell lines; 2MLN-dnTβRII, which expresses dominant negative form of TβRII, and control 2MLN-GFP, which expresses GFP. The transplanted tumors of these cell lines were subjected to gene expression analysis with Affymetrix U133 plus2 arrays.
 
Contributor(s) Komuro A, Yashiro M, Iwata C, Morishita Y, Johansson E, Matsumoto Y, Watanabe A, Aburatani H, Kiyono K, Shirai Y, Suzuki HI, Hirakawa K, Kano MR, Miyazono K
Citation(s) 19351925
Submission date Aug 05, 2008
Last update date Mar 25, 2019
Contact name Mitsunobu R Kano
E-mail(s) mikano-tky@umin.net
Phone +81-3-5841-3356
Fax +81-3-5841-3354
Organization name The University of Tokyo
Department Graduate School of Medicine
Lab Department of Molecular Pathology
Street address Hongo 7-3-1
City Bunkyo-ku
State/province Tokyo
ZIP/Postal code 113-0033
Country Japan
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (2)
GSM309953 2MLN_GFP
GSM309954 2MLN_dnTbRII
Relations
BioProject PRJNA113229

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Supplementary file Size Download File type/resource
GSE12336_RAW.tar 15.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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