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| Status |
Public on Nov 03, 2008 |
| Title |
Analysis of Aiolos and OBF-1 deletions in bone marrow from 7 week old mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The chromatin regulator Aiolos and the transcriptional coactivator OBF-1 have been implicated in regulating aspects of B cell maturation and activation. Mice lacking either of these factors have a largely normal early B cell development. However, when both factors are eliminated simultaneously a block is uncovered at the transition between pre-B and immature B cells, indicating that these proteins exert a critical function in developing B lymphocytes. In mice deficient for Aiolos and OBF-1, the numbers of immature B cells are reduced, small pre-BII cells are increased and a significant impairment in immunoglobulin light chain DNA rearrangement is observed. We identified genes whose expression is deregulated in the pre-B cell compartment of these mice. In particular, we found that components of the pre-BCR, such as the surrogate light chain genes l5l5 and VpreB, fail to be efficiently silenced in double-mutant mice. Strikingly, developmentally regulated nuclear repositioning of the l5l5 gene is impaired in pre-B cells lacking OBF-1 and Aiolos. These studies uncover a novel role for OBF-1 and Aiolos in controlling the transcription and nuclear organization of genes involved in pre-BCR function.
OBF-1 or Aiolos or both were deleted and the gene expression profiles for these animals investigated using Affymetrix arrays
Keywords: Genetic modifications
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| Overall design |
Two control wildtype animals, then duplicates for each of the single Aiolos or OBF-1 mutants and further duplicates for the Aiolos/OBF-1 double mutants
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| Contributor(s) |
Oakeley EJ, Karnowski A, Matthias P |
| Citation(s) |
18974788 |
| Submission date |
Aug 06, 2008 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Edward James Oakeley |
| E-mail(s) |
edward.oakeley@fmi.ch
|
| Organization name |
Friedrich Miescher Institute for Biomedical Research
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| Street address |
Maulbeerstrasse 66
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
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| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA113185 |
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