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| Status |
Public on May 02, 2019 |
| Title |
High-throughput combinatorial indexing enables scalable single-cell chromatin accessibility profiling [PBMC] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
While recent technical advancements have facilitated the mapping of epigenomes at the single-cell resolution, the throughput and quality of these methods have limited the widespread application of these technologies. Here, we describe a high-throughput platform for single-cell assay for transposase accessible chromatin (scATAC-seq) using droplet microfluidics that yields essential qualities for high-throughput profiling, including improvements in per-cell library complexity and throughput. This approach enables robust cell-typing of complex tissues and reveals a de novo atlas of cellular and epigenomic diversity across many cell types and cell stages.
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| |
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| Overall design |
Human peripheral blood mononuclear cells from 2 donors were processed using the BioRad SureCell scATAC-seq platform across multiple channels in the instrument.
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| |
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| Contributor(s) |
Lareau C |
| Citation(s) |
31235917 |
| Submission date |
Dec 10, 2018 |
| Last update date |
Aug 01, 2019 |
| Contact name |
Caleb Lareau |
| E-mail(s) |
lareauc@mskcc.org
|
| Organization name |
Memorial Sloan Kettering
|
| Street address |
417 E 68th St, Zuckerman - ZRC 1132
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE123581 |
Droplet-based combinatorial indexing for massive-scale single-cell chromatin accessibility |
|
| Relations |
| BioProject |
PRJNA509255 |
| SRA |
SRP173250 |
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