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Series GSE123669 Query DataSets for GSE123669
Status Public on Jun 26, 2019
Title Immature low-density neutrophils exhibit metabolic flexibility that facilitates breast cancer liver metastasis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Neutrophils play a key role in the control of metastatic progression. Neutrophils are phenotypically heterogeneous and can exert either anti- or pro-metastatic functions. Here, we demonstrate that tumor cells capable of forming liver metastases induce an accumulation of neutrophils in the peripheral blood and liver parenchyma. Cancer cell-derived G-CSF, in concert with other factors, mobilizes immature low-density neutrophils that promote liver metastasis. In contrast, mature high-density neutrophils inhibit the formation of liver metastases. Transcriptomic and metabolomic analyses of high- and low- density neutrophils reveal engagement of numerous metabolic pathways specifically in low-density neutrophils. Low-density neutrophils exhibit enhanced global bioenergetic capacity, through their ability to engage mitochondrial-dependent ATP production, and remain capable of executing pro-metastatic neutrophil functions, including NETosis, under nutrient-deprived conditions. Together, these data reveal that distinct pro-metastatic neutrophil populations exhibit a high degree of metabolic flexibility, which facilitates metastatic progression and the formation of liver metastases.
Overall design 4 samples of Naïve neutrophils (bone marrow), 4 samples HDNs (from peripheral blood of liver metastasis bearing mice), 4 samples LDNs (from peripheral blood of liver metastasis bearing mice)
Contributor(s) Siegel P, Hsu B
Citation(s) 31242422
Submission date Dec 11, 2018
Last update date Oct 08, 2019
Contact name Brian Hsu
Phone 5149935989
Organization name McGill University
Department Medicine
Lab Peter Siegel
Street address 1160 Pine Avenue West
City Montréal
State/province QC
ZIP/Postal code H3A1A3
Country Canada
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (12)
GSM3509270 HDN 3
GSM3509271 HDN 4
GSM3509272 HDN 5
BioProject PRJNA509476
SRA SRP173308

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE123669_HDN_vs_Naive_DEG.csv.gz 774.7 Kb (ftp)(http) CSV
GSE123669_LDN_vs_GSE123669_HDN_DEG.csv.gz 760.6 Kb (ftp)(http) CSV
GSE123669_LDN_vs_Naive_DEG.csv.gz 769.1 Kb (ftp)(http) CSV
GSE123669_Neutrophil_Cnts_070109.csv.gz 382.1 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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