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| Status |
Public on Dec 12, 2020 |
| Title |
Functional dissection of MIEN1 trans-regulation provides new evidence for colorectal cancer genome editing-based therapeutics |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
MIEN1 has been described as a key actor in colorectal cancer (CRC) biogenesis. We have used CRISPR/Cas9 technique to delete the promoter region of MIEN1 in HT-29 CRC cells. RNA-seq experiments revealed that several biological processes related to migration/metastasis were enriched by differentially expressed genes. Genetically modified cells shown decreased migration capacity.
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| Overall design |
RNA-seq experiments of WT and genetically modified HT-29 cells.
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| Contributor(s) |
Sierra-Diaz DC, Morel A, Ortega-Recalde O, Jiménez KM, Buitrago JC, Valero-Rubio D, Contreras NC, Baquero D, Barco A, Parra-Medina R, Monroy A, Figueroa C, Laissue P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 12, 2018 |
| Last update date |
Dec 14, 2020 |
| Contact name |
Oscar Javier Ortega-Recalde |
| E-mail(s) |
ojavieror@gmail.com
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| Organization name |
University of Otago
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| Department |
Anatomy
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| Street address |
60 Hanover Street
|
| City |
Dunedin |
| State/province |
Otago |
| ZIP/Postal code |
9016 |
| Country |
New Zealand |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA509662 |
| SRA |
SRP173340 |
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