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| Status |
Public on Oct 01, 2019 |
| Title |
The mithralog EC-7072 is highly cytotoxic to chronic lymphocytic leukemia cells by targeting the B-cell receptor signaling pathway |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
EC-7072, an analogue of Mithramycin A, exerts a direct cytotoxic effect on primary leukemic cells from patients with chronic lymphocytic leukemia (CLL) in vitro. To elucidate the underlying mechanisms mediating this effect, RNA sequencing was carried out employing total RNA from primary leukemic cells exposed to EC-7072. Data analysis revealed a dramatic impact of the compound on the transcriptional profile of CLL cells, unraveling a modulation of key mediators associated to homeostasis and survival of CLL cells, including B-cell receptor signaling. Likewise, EC-7072 activated the expression of a pro-apoptotic network, while downregulating the expression of essential anti-apoptotic proteins such as BCL2.
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| Overall design |
mRNA profiles of leukemic cells from 8 patients with chronic lymphocytic leukemia exposed to EC-7072
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| |
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| Contributor(s) |
Lorenzo-Herrero S, Sordo-Bahamonde C, Morís F, González S, López-Soto A |
| Citation(s) |
31681329 |
| Submission date |
Dec 13, 2018 |
| Last update date |
Nov 12, 2019 |
| Contact name |
Seila Lorenzo-Herrero |
| Organization name |
University of Oviedo
|
| Department |
Functional Biology
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| Lab |
Tumor Immunology
|
| Street address |
Julian Claveria
|
| City |
Oviedo |
| State/province |
Asturias |
| ZIP/Postal code |
33006 |
| Country |
Spain |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA509792 |
| SRA |
SRP173373 |
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