|
| Status |
Public on Dec 20, 2018 |
| Title |
Analysis of three kinds of tumors gene expression by RNA microarray |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In order to detect the difference of tumorigenicity induced by different cell lines in nude mice. We have employed whole genome microarray expression profiling as a discovery platform to identify the difference of genes expression. 10 week old 44 thymus-free nude mice were randomly divided into four groups, control group, A549 group, H1299 group and A549+H1299 group. 0.2ml saline suspension containing 5×10^6 cells were implanted subcutaneously in the flanks of each nude mouse in the control group. A549+H1299 group means 0.2ml saline suspension containing 2.5×10^6 A549 cells and 2.5×10^6 H1299 cells. After 30 days, Tumor tissue in each nude mouse Tumor tissue was taken out and detected with Agilent Human 4x44K Gene Expression Microarrays.
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| Overall design |
Gene expression in tumor tissue from A549, H1299 and A549+H1299 group were used for gene expression. Three independent experiments were performed at each group.
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| |
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| Contributor(s) |
Xu L, Ding B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 19, 2018 |
| Last update date |
Dec 22, 2018 |
| Contact name |
Li Xu |
| E-mail(s) |
xulihhb@163.com
|
| Phone |
13003691133
|
| Organization name |
ZJTCMU
|
| Street address |
BinWen Street 358#
|
| City |
HangZhou City |
| State/province |
ZheJiang Province |
| ZIP/Postal code |
310053 |
| Country |
China |
| |
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| Platforms (1) |
| GPL13497 |
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version) |
|
| Samples (9)
|
| GSM3521070 |
Tumor formation by H1299 cells-30day-rep1 |
| GSM3521071 |
Tumor formation by H1299 cells-30day-rep2 |
| GSM3521072 |
Tumor formation by H1299 cells-30day-rep3 |
| GSM3521073 |
Tumor formation by A549 cells-30day-rep1 |
| GSM3521074 |
Tumor formation by A549 cells-30day-rep2 |
| GSM3521075 |
Tumor formation by A549 cells-30day-rep3 |
| GSM3521076 |
Tumor formation by A549+H1299 cells-30day-rep1 |
| GSM3521077 |
Tumor formation by A549+H1299 cells-30day-rep2 |
| GSM3521078 |
Tumor formation by A549+H1299 cells-30day-rep3 |
|
| Relations |
| BioProject |
PRJNA510787 |
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