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| Status |
Public on Mar 23, 2023 |
| Title |
Transcription factor induction of vascular blood stem cell niches in vivo [ATAC-Seq] |
| Organism |
Danio rerio |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We report ATAC-seq data for FACS sorted zebrafish kdrl(flk1):mCherry+;mrc1a(1.3kb):GFP+ double positive cells collected from whole embryos at 72 hours post fertilization (hpf). We also report ATAC-seq data for the single positive fractions and the remaining, transgene negative, portion of the same embryos. The experiment was performed in triplicate.
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| Overall design |
kdrl:mCherry;mrc1a:GFP double positive transgenic embryos were homogenized, filtered, and sorted using FACS into PBS, collecting at least 12,000 cells (max 50,000) for each of the four cell populations.
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| Contributor(s) |
Hagedorn EJ, Yang S, Zon LI |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 19, 2018 |
| Last update date |
Mar 23, 2023 |
| Contact name |
Leonard Zon |
| E-mail(s) |
zon@enders.tch.harvard.edu
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| Organization name |
Boston Children's Hospital
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| Department |
Oncology/Hematology
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| Street address |
1 Blackfan Circle
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL18413 |
Illumina HiSeq 2500 (Danio rerio) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE124151 |
Transcription factor induction of vascular blood stem cell niches in vivo |
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| Relations |
| BioProject |
PRJNA510837 |
| SRA |
SRP173968 |
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