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| Status |
Public on Mar 23, 2023 |
| Title |
Transcription factor induction of vascular blood stem cell niches in vivo [TOMO-Seq] |
| Organism |
Danio rerio |
| Experiment type |
Other
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| Summary |
We report RNA tomography (tomo-seq) gene expression data for the tail region of a 72 hpf zebrafish embryo. The portion of the tail containing the caudal hematopoietic tissue (CHT) was isolated by manual dissection, snap froze, and then cryosections (40 in total, 8 µm-thick) were collected along the dorsal-ventral axis. Each cryosection was placed into a tube and the RNA was exctracted and barcoded during a reverse trancription step prior to library synthesis and sequencing.
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| Overall design |
The region of the tail containing the CHT was isolated using a scalpel and then snap-frozen on dry ice. The RNA from individual cryosections was extracted using Trizol reagent and then barcoded during a reverse transcription step prior to library synthesis according to the previously published method (Junker et al., 2014).
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| Contributor(s) |
Hagedorn EJ, Junker J, Yang S, Zon LI |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 19, 2018 |
| Last update date |
Mar 23, 2023 |
| Contact name |
Leonard Zon |
| E-mail(s) |
zon@enders.tch.harvard.edu
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| Organization name |
Boston Children's Hospital
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| Department |
Oncology/Hematology
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| Street address |
1 Blackfan Circle
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL18413 |
Illumina HiSeq 2500 (Danio rerio) |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE124151 |
Transcription factor induction of vascular blood stem cell niches in vivo |
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| Relations |
| BioProject |
PRJNA510841 |
| SRA |
SRP173971 |
