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| Status |
Public on Mar 12, 2019 |
| Title |
Dlk1-Mediated Temporal regulation of Notch Signaling is Required for Differentiation of Alveolar Type II to Type I Cells during Lung Repair |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived retinal transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis
Methods: Lung epithelia cells were collected after 10 days of treatment by pseudomonas aeruginosa (PA), RNA-Seq and qRT-PCR were applied to analyze the high-throughput data
Results: In general, the mutant type II-like cells expressed higher levels of type I cell markers and lower levels of type II cell markers
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| Overall design |
Total RNA profiles of mutant(SpC-CreER/Dlk1flox/ROSA-Tomato) and wild-type(SpC-CreER/ROSA-Tomato) mice were generated by deep sequencing, in triplicate, using Illumina HiSeq4000.
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| Contributor(s) |
Liu Y, Zhang W, Malik A |
| Citation(s) |
30865885 |
| Submission date |
Dec 21, 2018 |
| Last update date |
Mar 19, 2019 |
| Contact name |
Issam Awad |
| E-mail(s) |
iawad@uchicago.edu
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| Phone |
773-702-6222
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| Organization name |
The University of Chicago
|
| Department |
Department of Neurology
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| Lab |
Issam Awad
|
| Street address |
5841 S. Maryland Avenue
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA511430 |
| SRA |
SRP174200 |
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