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Series GSE124540 Query DataSets for GSE124540
Status Public on Jun 22, 2021
Title ChIP-seq and RNA-seq analysis of histone Kcr in the embryonic forebrain and neural stem/progenitor cells.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary To gain a deeper insight into how histone lysine crotonylation regulates embryonic neural stem/progenitor cells (NSPCs) proliferation and differentiation, ChIP-seq and RNA-seq were performed to analyze the genome-wide changes of histone Kcr and transcriptiome changes stimulated with crotonate and MS-275 in E13.5 NSPCs.
 
Overall design Total RNA was extracted from E13.5 NSPCs before and after crotonate and MS-275 stimulation and generated by deep sequencing, in four biological replicates, using Illumina novaseq 6000. ChIP was excuted using pan-Kcr and Pan-Kac antibody with E13.5 NSPCs before and after crotonate and MS-275 stimulation.
 
Contributor(s) Dai S, Liu C
Citation(s) 34369651
Submission date Jan 02, 2019
Last update date Aug 10, 2021
Contact name Dai Shang Kun
E-mail(s) daisk@sdut.edu.cn
Organization name Shandong University of Technology
Department School of Life Sciences and Medicine
Lab Laboratory of Developmental and Evolutionary Biology
Street address 266 Xincun West Road
City Zibo
State/province Shandong
ZIP/Postal code 255000
Country China
 
Platforms (2)
GPL23479 BGISEQ-500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (30)
GSM3536156 Transcriptome_E13.5_NSPCs_Control-rep1_RNA-seq
GSM3536157 Transcriptome_E13.5_NSPCs_Control-rep2_RNA-seq
GSM3536158 Transcriptome_E13.5_NSPCs_Control-rep3_RNA-seq
Relations
BioProject PRJNA512461
SRA SRP174993

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE124540_E13.5_Brain_Explant_RNA-seq_Crotonate-Control_DEG.txt.gz 1.9 Mb (ftp)(http) TXT
GSE124540_H3K9ac_E13.5_Forebrain_Peaks.bed.gz 417.3 Kb (ftp)(http) BED
GSE124540_H3K9cr_E13.5_Forebrain_Peaks.bed.gz 282.4 Kb (ftp)(http) BED
GSE124540_Histone-Kac_Control_E13.5_NSPCs_Peaks.bed.gz 184.4 Kb (ftp)(http) BED
GSE124540_Histone-Kac_Crotonate_E13.5_NSPCs_Peaks.bed.gz 172.6 Kb (ftp)(http) BED
GSE124540_Histone-Kac_MS-275_E13.5_NSPCs_Peaks.bed.gz 62.1 Kb (ftp)(http) BED
GSE124540_Histone-Kcr_Control_E13.5_NSPCs_Peaks.bed.gz 140.8 Kb (ftp)(http) BED
GSE124540_Histone-Kcr_Crotonate_E13.5_NSPCs_Peaks.bed.gz 184.5 Kb (ftp)(http) BED
GSE124540_Histone-Kcr_MS-275_E13.5_NSPCs_Peaks.bed.gz 48.9 Kb (ftp)(http) BED
GSE124540_RAW.tar 197.8 Mb (http)(custom) TAR (of BW)
GSE124540_RNA-seq_TPM.txt.gz 1.4 Mb (ftp)(http) TXT
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