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Series GSE124778 Query DataSets for GSE124778
Status Public on Jul 26, 2019
Title TRIM33 deficiency in mature myeloid cells impairs resolution of colonic inflammation
Organism Mus musculus
Experiment type Expression profiling by array
Summary Mature myeloid cells play a crucial role in the pathogenesis of Crohn disease (CD) but the molecular players that regulate their functions in CD are not fully characterized. Here we show that Trim33 mRNA level is decreased in CD patient’s blood monocytes and characterize TRIM33 functions in monocytes during dextran sulfate sodium (DSS) induced colitis. Mice deleted for trim33 only in mature myeloid cells (Trim33-/- mice) display an impaired resolution of colonic inflammation. This deficiency is associated with an increased number of blood and colon neutrophils and monocytes and a decreased number of colonic macrophages. In accordance, Trim33-/- monocytes are less competent that wild type monocytes for recruitment and differentiation into macrophages at the inflammatory site. Furthermore, during resolution of DSS-induced colitis, Trim33-/- colonic macrophages display an impaired M1/M2 switch and express a low level of membrane bound TNFα known to regulate resolution of inflammation. Altogether, these results show an important role of TRIM33 in monocytes/macrophages during the resolution of DSS-induced colonic inflammation and pinpoint TRIM33 as a novel Crohn disease biomarker and as a potential therapeutic target.
 
Overall design monocytes cells cells from blood of C57BL/6 LyzCre TRIM33 mice vs WT mice sorted as CD11b+ CD115+ Ly6C high for Tmem using a FACS-ARIA3 flow cytometer.
 
Contributor(s) PARCELIER A, MATHE C, BARROCA V, TORRES C, FERRI F, LAWANDOWSKI D, DINET O, BOSCHETTI G, PETIT V, ROMEO P
Citation(s) 31130476
Submission date Jan 08, 2019
Last update date Jul 26, 2019
Contact name Franck Letourneur
E-mail(s) franck.letourneur@inserm.fr
Organization name INSERM
Department U1016
Lab genomic
Street address 22 rue mechain
City Paris
ZIP/Postal code 75014
Country France
 
Platforms (1)
GPL18802 [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [mogene20st_Mm_ENTREZG_18.0.0]
Samples (12)
GSM3553938 178
GSM3553939 TRIM33_mix_1
GSM3553940 TRIM33_mix_2
Relations
BioProject PRJNA513468

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE124778_RAW.tar 117.0 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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