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| Status |
Public on Jun 06, 2019 |
| Title |
EOMES-dependent epigenetic reprogramming during CD8 T cell development leads to acquisition of memory traits (RNA-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Memory CD8 T cells have a unique ability to provide lifelong immunity against pathogens. Although memory features generally arise after a challenge with foreign antigen, naïve CD8 single positive (SP) thymocytes may already acquire phenotypic and functional characteristics of memory cells in response to cytokines such as interleukin-4. This process is associated with the induction of the T-box transcription factor Eomesodermin (EOMES). However, the underlying molecular mechanisms remain ill-defined. Using epigenomic profiling, we show that these “innate memory” CD8SP cells acquire only a portion of the active enhancer repertoire of conventional memory cells. This reprograming was secondary to EOMES recruitment, mostly to RUNX3-bound enhancers. Furthermore, we identified direct interaction between EOMES and BRG1 and showed that in vivo acquisition of EOMES-dependent program by CD8SP thymocytes was dependent on this chromatin remodeling factor. In conclusion, our results support a strong epigenetic basis for EOMES-driven establishment of CD8 T cell unconventional memory program.
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| |
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| Overall design |
CD8SP thymocytes: Innate Memory, Naïve, WT, and Eomes-TG
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| |
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| Contributor(s) |
Istaces N, Splittgerber M, Silva VL, Nguyen M, Thomas S, Le A, Achouri Y, Calonne E, Defrance M, Fuks F, Goriely S, Azouz A |
| Citation(s) |
31341159 |
| Submission date |
Jan 10, 2019 |
| Last update date |
Sep 23, 2019 |
| Contact name |
Stanislas Goriely |
| E-mail(s) |
stgoriel@ulb.ac.be
|
| Phone |
+3226509586
|
| Organization name |
Université Libre de Bruxelles
|
| Lab |
IMI
|
| Street address |
Rue Adrienne Bolland 8
|
| City |
Gosselies |
| ZIP/Postal code |
B-6041 |
| Country |
Belgium |
| |
|
| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE124914 |
EOMES-dependent epigenetic reprogramming during CD8 T cell development leads to acquisition of memory traits |
|
| Relations |
| BioProject |
PRJNA514301 |
| SRA |
SRP178227 |
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