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| Status |
Public on Jan 10, 2020 |
| Title |
Bone marrow microenvironment mediated drug resistance in Acute promyelocytic leukemia |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
About 5-10% newly diagnosed and about 20-30% of relapsed acute promyelocytic leukemia (APL) patients will have disease recurrence after receiving currently accepted standards of care. While there are reports of micro-environment mediated drug resistance (EM-DR) in AML, there is no data on the effect of malignant promyelocyte and stromal interaction on Arsenic trioxide (ATO) induced apoptosis. There are limited study available on the effect of leukemic cell interaction on stromal cells. We undertook a preliminary study to evaluate the changes induced by leukemic cells on stromal cells. In a gene expression profiling comparing HS-5 cells in co-culture with NB4 cells alone, 8456 genes were differentially regulated. On a preliminary analysis, we observed an up-regulation of various pathways such as adhesion, Cytokines, Wnt signalling in the stromal cells.
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| Overall design |
The HS-5 cells were co-cultured with NB4 cells for 48 hours and the stromal cells were taken out after washing with PBS, characterized by flow cytometry to obstain a purity of more than 95% and subjected to RNA isolation. Similarly HS-5 cells alone were alone used as controls
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| Contributor(s) |
Mathews V, Ganesan S, Kumar Palani H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 11, 2019 |
| Last update date |
Jan 12, 2020 |
| Contact name |
Genotypic technology |
| E-mail(s) |
sudha.rao@genotypic.co.in
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| Organization name |
Genotypic Technology
|
| Street address |
259, Apoorva 4th cross,80 feet Road,RMV 2ND STAGE
|
| City |
Bangalore |
| State/province |
Karnataka |
| ZIP/Postal code |
560094 |
| Country |
India |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA514741 |
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