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| Status |
Public on Feb 01, 2009 |
| Title |
Oct4-Induced Pluripotency in Adult Neural Stem Cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The four transcription factors Oct4, Sox2, Klf4, and c-Myc can induce pluripotency in mouse and human fibroblasts. We previously described direct reprogramming of adult mouse neural stem cells (NSCs) by Oct4 and either Klf4 or c-Myc. NSCs endogenously express Sox2, c-Myc, and Klf4 as well as several intermediate reprogramming markers. Here we report that exogenous expression of the germline-specific transcription factor Oct4 is sufficient to generate pluripotent stem cells from adult mouse NSCs. These one-factor induced pluripotent stem (1F iPS) cells are similar to embryonic stem cells in vitro and in vivo. Not only can these cells be efficiently differentiated into NSCs, cardiomyocytes and germ cells in vitro, but they are also capable of teratoma formation and germline transmission in vivo. Our results demonstrate that Oct4 is required and sufficient to directly reprogram NSCs to pluripotency.
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| Overall design |
10 hybridizations in total.
NSC-derived iPS cells by one-factor (Oct4) in triplicate:
- NSC_1F_iPS_1
- NSC_1F_iPS_2
- NSC_1F_iPS_3
One-factor (Oct4) iPS cell-derived NSC in triplicate:
- 1F_iPS_NSC_1
- 1F_iPS_NSC_2
- 1F_iPS_NSC_3
Neural stem cell (NSC) derived from brain of OG2/Rosa26 mice:
- NSC_1
- NSC_2
- NSC_3
- NSC_4
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| Contributor(s) |
Kim J, Sebastiano V, Arauzo-Bravo MJ, Sasse P, Wu G, Gentile L, Ko K, Ruau D, Ehrich M, van den Boom D, Ortmeier C, Zenke M, Fleischmann BK, Zaehres H, Schöler HR |
| Citation(s) |
19203577 |
| Submission date |
Aug 20, 2008 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Martin Zenke |
| E-mail(s) |
Martin.Zenke@rwth-aachen.de
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| Phone |
+49-241-80 80760
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| Organization name |
Institute for Biomedical Engineering
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| Department |
Cell Biology
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| Street address |
Universitatsklinikum Aachen, RWTH
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| City |
Aachen |
| State/province |
NRW |
| ZIP/Postal code |
52074 |
| Country |
Germany |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (10)
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| GSM314038 |
NSCs-derived iPS cells by one-factor (Oct4) sample_1 |
| GSM314039 |
NSCs-derived iPS cells by one-factor (Oct4) sample_2 |
| GSM314040 |
NSCs-derived iPS cells by one-factor (Oct4) sample_3 |
| GSM314042 |
One-factor (Oct4) iPS cell-derived NSCs sample_1 |
| GSM314043 |
One-factor (Oct4) iPS cell-derived NSCs sample_2 |
| GSM314044 |
One-factor (Oct4) iPS cell-derived NSCs sample_3 |
| GSM314045 |
Neural stem cell sample_1 |
| GSM314046 |
Neural stem cell sample_2 |
| GSM314047 |
Neural stem cell sample_3 |
| GSM314048 |
Neural stem cell sample_4 |
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| Relations |
| BioProject |
PRJNA112981 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE12499_RAW.tar |
39.9 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
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