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Series GSE125187 Query DataSets for GSE125187
Status Public on Jun 14, 2021
Title Gene expression data in Control, Doxorubicin-resistant, and Epirubicin-resistant breast cancer cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Development of chemotherapy resistance is a critical barrier in cancer treatment. Increased reliance on mitochondrial metabolism has been described as a distinctive characteristic of resistant cancers, however it is unknown whether enhanced oxidative metabolism is an intrinsic property or whether the metabolic signature of resistant cancers is dependent on the therapeutics. In this study, we used microarrays to detail the differences in global gene expression between anthracycline-resistant and -sensitive breast cancer cells. Taken together with metabolomics, bioenergetics, and functional experiments, we show that two anthracyclines (doxorubicin and epirubicin) elicit distinct metabolic adaptations in breast cancer and that interfering with global metabolic regulators can overcome resistance to different therapeutic agents by their ability to simultaneously interfere with numerous metabolic pathways.
Overall design We obtained MCF-7 breast cancer cells selected in increasing doses of doxorubicin or epirubicin, previously described in PMID 18980695 and GSE12115. Cells were cultured using the same media and grown at similar densities. Control (sensitive) cells were maintained in 0.1% DMSO, DoxR (doxorubicin-resistant) cells in 98.1 nM doxorubicin, and EpiR (epirubicin-resistant) cells in 852 nM epirubicin at all times. Each condition has 3 biological replicates.
Contributor(s) McGuirk S, St-Pierre J
Citation(s) 34181531
Submission date Jan 16, 2019
Last update date Jul 07, 2021
Contact name Julie St-Pierre
Phone 6135625800-3777
Organization name University of Ottawa
Department Department of Biochemistry, Microbiology and Immunology
Lab 4210
Street address 451 Smyth Road
City Ottawa
State/province Ontario
ZIP/Postal code K1H8M5
Country Canada
Platforms (1)
GPL16686 [HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version]
Samples (9)
GSM3564825 Control cells, biological rep 1
GSM3564826 Control cells, biological rep 2
GSM3564827 Control cells, biological rep 3
BioProject PRJNA515492

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE125187_RAW.tar 72.4 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data provided as supplementary file
Processed data included within Sample table

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