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| Status |
Public on Jan 18, 2019 |
| Title |
A Novel Glioma-specific Enhancer Maintains Glioma Temozolomide Sensitivity By Regulation of RasGRP3 Expression via Long-range Chromosome Interactions |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
In this study, we report the identification of a glioma-specific enhancer that confers sensitivity to TMZ by upregulation of the mitogen-associated protein kinase activator RasGRP3. Deletion of either the active domain of RasGPR3 or the enhancer using CRISPR/Cas9, was sufficient to restore sensitivity to TMZ in a resistant population of cells that was reversed with overexpression of RasGRP3. The project include RNA-seq, Hi-C, Capture Hi-C between DMSO-treated U251 and TMZ-treated U251.
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| |
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| Overall design |
RNA-seq, Hi-C, Capture Hi-C between DMSO-treated U251 and TMZ-treated U251
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| |
|
| Contributor(s) |
Chen B, Lu W |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| BioProject |
PRJNA515368 |
| Submission date |
Jan 17, 2019 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Bohan Chen |
| E-mail(s) |
bhchen1@sina.cn
|
| Organization name |
Nankai University
|
| Street address |
NO.94 Weijin Road
|
| City |
Tianjin |
| ZIP/Postal code |
300071 |
| Country |
China |
| |
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| Platforms (2) |
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| Samples (12)
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| Relations |
| SRA |
SRP179691 |
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