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| Status |
Public on Jun 26, 2020 |
| Title |
Genome binding/occupancy profiling of Smad4 in Th17 cells cultured at 37°C and 39.5°C after 24 hours. |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Smad4 was required for febrile temperature enhanced Th17 differentiation. In order to further understand the mechanisms whereby Smad4 enhance Th17 differentiation, we conducted ChIP-seq analysis of Smad4 in Th17 cells cutured 37°C and 39.5°C after 24 hours.
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| Overall design |
Naïve CD4+ T cells were sorted from wild type mice and differentiated under IL-6 plus TGF-β1 condition at 37°C and 39.5°C for 24 hours. The collected cells were then subjected to ChIP and construction of library for ChIP-seq.
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| Contributor(s) |
Ni L, Zhao X |
| Citation(s) |
32049050 |
| Submission date |
Jan 17, 2019 |
| Last update date |
Sep 25, 2020 |
| Contact name |
Chen Dong |
| Organization name |
Tsinghua University
|
| Department |
Institute for Immunology and School of Medicine
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| Lab |
Dongchen's lab
|
| Street address |
Haidian District
|
| City |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
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| Platforms (1) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE125265 |
ChIP-Seq of Smad4 in Th17 cells cultured at 37°C and 39.5°C and RNA-Seq of wild type and Smad4-deficient Th17 cells cultured at 37°C and 39.5°C |
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| Relations |
| BioProject |
PRJNA515714 |
| SRA |
SRP179992 |
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