|
| Status |
Public on Jun 11, 2009 |
| Title |
Wheat "Chinese Spring" natural antisense transcription survey |
| Organism |
Triticum aestivum |
| Experiment type |
Expression profiling by array
|
| Summary |
The Affymetrix GeneChip Wheat Genome Array currently provides the most comprehensive coverage of the wheat genome for a microarray. In addition to using this resource for transcript expression studies and hybridization-based DNA marker discovery, we endeavored to use the GeneChip to discover the expression of natural antisense transcript (NAT) pairs. By using alternative target preparation schemes, both the sense- and antisense-strand derived transcripts were labeled and hybridized to the Wheat GeneChip. To enable maximum discovery, five different tissue types were selected for assay, and the wheat cultivar ‘Chinese Spring’ was used considering that most of the GeneChip probe sequences were based on sequencing of this genome. [PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Tristan Coram. The equivalent experiment is TA21 at PLEXdb.]
|
| |
|
| Overall design |
target strand labeled: Sense(1-replications); target strand labeled: Antisense(1-replications)
|
| Web link |
http://www.plexdb.org/modules/PD_browse/experiment_browser.php?experiment=TA21
|
| |
|
| Contributor(s) |
Coram T |
| Citation(s) |
19480707 |
| Submission date |
Aug 22, 2008 |
| Last update date |
Dec 04, 2018 |
| Contact name |
PLEXdb Curator, Sudhansu Dash |
| Organization name |
Iowa State University
|
| Department |
Virtual Reality Application Center
|
| Lab |
PLEXdb
|
| Street address |
1023 Crop Genomes Informatics Lab (Iowa State University)
|
| City |
Ames |
| State/province |
IA |
| ZIP/Postal code |
50011 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL3802 |
[wheat] Affymetrix Wheat Genome Array |
|
| Samples (2) |
| GSM315202 |
target strand labeled: Sense - rep1 |
| GSM315203 |
target strand labeled: Antisense - rep1 |
|
| Relations |
| BioProject |
PRJNA112935 |
