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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 19, 2019 |
| Title |
miRNA-Seq of testicular peritubular myoid cells, other testicular cells, and other tissue smooth muscle-like cells |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
In addition to germ cell population, testes contain several types of somatic cells, including Sertoli cells, Leydig cells, and peritubular myoid (PTM) cells. PTM cells are a type of smooth muscle-like cells and have contraction ability. To identify a marker of PTM cells, we isolated primary cells and subsequently performed miRNA-Seq of testicular peritubular myoid cells (PTM_1, PTM_2, PTM_3), testicular Sertoli cells (Sertoli_1, Sertoli_2, Sertoli_3), testicular Leydig cells (Leydig_1, Leydig_2, Leydig_3), testicular germ cells (Germ_1, Germ_2, Germ_3), vascular smooth muscle cells (VSMC_1, VSMC_2, VSMC_3), intestinal smooth muscle cells (ISMC_1, ISMC_2, ISMC_3), and tracheal smooth muscle cells (TSMC_1, TSMC_2, TSMC_3) using BGISEQ-500 platform (BGI, China).
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| Overall design |
The mouse testicular cells were isolated as previously described (PMID: 23759306, 26995740, 30306767). Smooth muscle-like cells were obtaiend from Procell (Wuhan, China).
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| Contributor(s) |
Chen S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 18, 2019 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Su-Ren Chen |
| E-mail(s) |
chensuren@ioz.ac.cn
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| Organization name |
Institute of Zoology, Chinese Academy of Sciences
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| Department |
State Key Laboratory of Stem Cell and Reproductive Biology
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| Street address |
1Beichen West Road, Chaoyang District
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| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (1) |
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| Samples (21)
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| This SubSeries is part of SuperSeries: |
| GSE125304 |
RNA-seq and miRNA-seq of testicular peritubular myoid cells, other testicular cells, and other tissue smooth muscle-like cells |
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| Relations |
| BioProject |
PRJNA515822 |
| SRA |
SRP180144 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE125302_miR-abundance_values_for_ALL_samples.xlsx |
10.4 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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