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| Status |
Public on Feb 01, 2020 |
| Title |
Gut microbiota mediates intermittent-fasting alleviation of diabetes-induced cognitive impairment |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We reported a 28-day Intermittent fasting (IF) regimen improved cognitive deficits in db/db mice via a microbiota-metabolites-brain axis assessed by behavioral tests and multiple-omics analysis (transciptomics, 16S rRNA sequencing and metabolomics). Here we present transcriptomics data of mice hippocampus. A total of 310.85Gb clean RNA-SEQ reads of all mice with an average depth of 3.86x were obtained and were then mapped against the Mus musculus genome to obtain the gene expression FPKM values for each sample. We detected 27,094 genes (including 1,345 new predicted genes with no annotation) with FPKM value. Among them, 1,181 genes were found to be only highly expressed in db/db-IF mice compared to db/db and db/m mice, using Differentially Expressed Genes (DEG) analysis, most of which enriched in mitochondrial-related GO terms. Besides, IF strongly elevated genes related to KEGG pathway of oxidative phosphorylation (OXPHOS) via up-regulating mitochondrial located genes expression. In consistent with results from RNA-sequencing analysis, the qPCR analysis confirmed that mitochondrial and metabolic genes expressed were upregulated by IF in db/db mice. In conclusion, IF regimen significantly enhanced mitochondrial and energy metabolism related genes expressions in diabetic mice hippocampus.
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| Overall design |
11 db/m mice,10 db/db mice,10 db/db-IF mice
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| Contributor(s) |
Liu Z, Jin X |
| Citation(s) |
32071312 |
| Submission date |
Jan 21, 2019 |
| Last update date |
May 07, 2020 |
| Contact name |
Jin Xin |
| E-mail(s) |
jinxin1@genomics.cn
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| Phone |
13419631327
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| Organization name |
BGI
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| Street address |
Beishan road
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| City |
Shenzhen |
| State/province |
Guangdong |
| ZIP/Postal code |
518120 |
| Country |
China |
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| Platforms (1) |
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| Samples (31)
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| Relations |
| BioProject |
PRJNA516195 |
| SRA |
SRP181024 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE125387_FPKM.xlsx.gz |
9.7 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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