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| Status |
Public on Jan 11, 2022 |
| Title |
A Mediator-cohesin axis controls heterochromatin domain formation |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
The genome consists of regions of transcriptionally active euchromatin and more silent heterochromatin. We reveal that the formation of heterochromatin domains requires cohesin turnover on DNA. Stabilization of cohesin on DNA through depletion of its release factor WAPL leads to a near-complete loss of heterochromatin domains. The Mediator CDK module controls heterochromatin in an opposing manner. Loss of this module leads to an almost binary partition of the genome into dense H3K9me3 domains, and regions devoid of H3K9me3 spanning the rest of the genome. By restricting the degree of heterochromatinization, the Mediator CDK module creates a transcription-permissive context that enables gene expression within heterochromatin domains. WAPL deficiency prevents the formation of heterochromatin domains, thus allowing for gene expression even in the absence of the Mediator CDK module. We propose that heterochromatin is controlled by two opposing activities, in which the Mediator CDK module and cohesin turnover must be balanced to enable the correct distribution of epigenetic marks to ensure proper gene expression.
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| Overall design |
Hi-C
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| Contributor(s) |
van der Weide RH, de Wit E |
| Citation(s) |
35136067 |
| Submission date |
Jan 24, 2019 |
| Last update date |
Feb 23, 2022 |
| Contact name |
Robin H. van der Weide |
| Organization name |
Hubrecht Institute
|
| Lab |
Kind Lab
|
| Street address |
Uppsalalaan 8
|
| City |
Utrecht |
| State/province |
Utrecht |
| ZIP/Postal code |
3584 CT |
| Country |
Netherlands |
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| Platforms (1) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE125672 |
A Mediator-cohesin axis controls heterochromatin domain formation. |
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| Relations |
| BioProject |
PRJNA516941 |
| SRA |
SRP181954 |
