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Status |
Public on Nov 25, 2019 |
Title |
The effect pregnane receptor X (PXR) agonist PCN on hepatic gene expression in mouse liver with and without glucose |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
The mice were treated i.p. with pregnenolone-16-α-carbonitrile (PCN, 50mg/kg dissolved in DMSO-corn oil 1:3) or vehicle (DMSO-corn oil 1:3) once daily for four days. The mice were fasted for four hours and then administered glucose (2g/kg) by oral gavage or further kept on fasting. The mice were sacrificed 1 hour after glucose administration.
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Overall design |
The effect of prototypical pregnane receptor X (PXR) agonist (pregnenolone 16α-carbonitrile) PCN on hepatic gene expression was studied in male C57BL/6 mice. PCN and DMSO-corn oil (30%:70%) as a control were given for 4 days. After 6 hours of fasting, glucose (2 mg/g) was given orally. After one hour mice were sacrificed and tissues were collected. Total RNA isolated from mouse liver tissue
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Contributor(s) |
Hakkola J, Rysä J |
Citation(s) |
31723190 |
Submission date |
Jan 25, 2019 |
Last update date |
Nov 25, 2019 |
Contact name |
Jaana Rysae |
E-mail(s) |
jaana.rysa@gmail.com
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Organization name |
University of Eastern Finland
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Department |
School of Pharmacy
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Street address |
Yliopistonranta 1C
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City |
Kuopio |
ZIP/Postal code |
70211 |
Country |
Finland |
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Platforms (1) |
GPL21278 |
Illumina Sentrix BeadChip Mouse WG-6 v2 array [Probe ID version] |
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Samples (12)
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Relations |
BioProject |
PRJNA517179 |