 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 29, 2019 |
| Title |
Nano genome altas (NGA) of body wide tissue specific responses |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: In order to detect the unpredictable risks of the cumulative nanoparticles infiltrated everywhere in the whole body after systematic administration
Methods: By using RNA-seq, we systematically studied the transcriptomic response of multi-organs from mice and tissue specific (stem) cells to the hydroxyapatite (HA) nano-delivery system.
Results: We show that HA could differentially influence gene expression, immune response, cell proliferation, stress, apoptosis, metabolic, ion transport activity, cell signaling and epigenetic activity across tissues and tissue specific (stem) cells.
|
| |
|
| Overall design |
We collected mouse tissues of heart, liver, spleen, kidney, skeletal muscle, peripheral blood mononuclear cells (PBMC) as well as tissue specific (stem) cells of mouse skeletal muscle derived stem cells (MUSC), Human bone marrow derived stem cells (hBMSC), Human primary umbilical vein endothelial cell lines (HUVEC) for RNA-seq after treated with the two groups (PBS control and HA solution) for 24hours, respectively.
|
| |
|
| Contributor(s) |
Wu B, Zou X, Ouyang H |
| Citation(s) |
30903824 |
| Submission date |
Jan 28, 2019 |
| Last update date |
Mar 29, 2019 |
| Contact name |
Bingbing Wu |
| E-mail(s) |
yingxianaa@163.com
|
| Phone |
+86-571-13735548673
|
| Organization name |
Zhejiang University
|
| Department |
School of Medcine
|
| Lab |
Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine
|
| Street address |
866 Yuhangtang Rd
|
| City |
HangZhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310058 |
| Country |
China |
| |
|
| Platforms (2) |
|
| Samples (58)
|
|
| Relations |
| BioProject |
PRJNA517433 |
| SRA |
SRP182662 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE125742_human-counts-5-23-ID.xlsx |
3.4 Mb |
(ftp)(http) |
XLSX |
| GSE125742_mice-counts-5-23-ID.xlsx |
5.0 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...