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Series GSE125788 Query DataSets for GSE125788
Status Public on May 17, 2021
Title Transcription profiling of IL-4-expressing CD4 T cells in the lung and lung-draining lymph nodes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Using single cell RNA-sequencing and whole-genome targeted transcription profiling, this study reveals that helminth infection causes the expansion of distinct but clonally related IL-4-competent CD4+ T cells in the lung and lung-draining lymphoid tissues providing insight into tissue-specific mechanisms of type-2 cytokine regulation and the lineage commitment of T-helper 2 (Th2) and T follicular helper (Tfh) cells.
 
Overall design Genome-wide targeted sequencing to profile transcription of 4 distinct CD4+ T cells in lymphoid and nonlymphoid tissues
 
Contributor(s) Reinhardt RL, Seibold MA, Dyjack N
Citation(s) 34106992
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 AI119004 The Role of BATF in Allergic Inflammation and Anti-Helminth Immunity NATIONAL JEWISH HEALTH Richard Lee Reinhardt
R01 AI156901 The Origin and Role of Pulmonary ILC2 Subsets in Anti-Helminth Immunity NATIONAL JEWISH HEALTH Richard Lee Reinhardt
Submission date Jan 28, 2019
Last update date Aug 16, 2021
Contact name Richard Lee Reinhardt
E-mail(s) reinhardtl@njhealth.org
Organization name National Jewish Health
Department Biomedical Research
Lab Reinhardt
Street address 1400 Jackson Street, K817A
City Denver
State/province Colorado
ZIP/Postal code 80206
Country USA
 
Platforms (2)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
GPL25723 Ion Torrent S5 (Mus musculus)
Samples (29)
GSM3581882 IL4-positive lymph node
GSM3581883 IL4-negative lymph node
GSM3581884 IL-4-positive Lung
Relations
BioProject PRJNA517508
SRA SRP182726

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE125788_10x_rawData.txt.gz 5.5 Mb (ftp)(http) TXT
GSE125788_10x_scaledData.txt.gz 283.9 Mb (ftp)(http) TXT
GSE125788_124.18-04-18.S5XL-00648.bcmatrix.txt.gz 381.8 Kb (ftp)(http) TXT
GSE125788_139.19-09-18.S5XL-00648.bcmatrix.txt.gz 659.5 Kb (ftp)(http) TXT
GSE125788_144.21-09-18.S5XL-00648.bcmatrix.txt.gz 671.5 Kb (ftp)(http) TXT
GSE125788_145.21-09-18.S5XL-00648.bcmatrix.txt.gz 666.6 Kb (ftp)(http) TXT
GSE125788_RawCount_Matrix.txt.gz 706.5 Kb (ftp)(http) TXT
GSE125788_countNorm_Matrix.txt.gz 2.6 Mb (ftp)(http) TXT
GSE125788_vstNorm_Matrix.txt.gz 2.7 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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