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| Status |
Public on Nov 05, 2019 |
| Title |
Modular transcriptional programs separately define axon and dendrite connectivity |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Patterns of synaptic connectivity are remarkably precise and complex. Single-cell RNA sequencing has revealed a vast transcriptional diversity of neurons. Nevertheless, a clear logic underlying the transcriptional control of neuronal connectivity has yet to emerge. Here, we focused on Drosophila T4/T5 neurons, a class of closely related neuronal subtypes with different wiring patterns. Eight subtypes of T4/T5 neurons are defined by combinations of two patterns of dendritic inputs and four patterns of axonal outputs. Single-cell profiling during development revealed distinct transcriptional programs defining each dendrite and axon wiring pattern. These programs were defined by the expression of a few transcription factors and different combinations of cell surface proteins. Gain and loss of function studies provide evidence for independent control of different wiring features. We propose that modular transcriptional programs for distinct wiring features are assembled in different combinations to generate diverse patterns of neuronal connectivity.
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| Overall design |
Single-cell profiling of FACS purified populations of Drosophila melanogaster T4/T5 neurons during pupal development (24h and 48h APF).
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| Contributor(s) |
Kurmangaliyev YZ, Zipursky LS |
| Citation(s) |
31687928 |
| Submission date |
Feb 06, 2019 |
| Last update date |
Sep 21, 2020 |
| Contact name |
Yerbol Z. Kurmangaliyev |
| Organization name |
University of California, Los Angeles
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| Street address |
675 Charles E. Young Drive South
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| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL21306 |
Illumina HiSeq 4000 (Drosophila melanogaster) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA521120 |
| SRA |
SRP184201 |
