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| Status |
Public on Jul 31, 2019 |
| Title |
Androgen receptor is a back-door inhibitor of Androgen Receptor is a Backdoor Inhibitor of Wildtype and Mutant Estrogen Receptors in Refractory Hormone Receptor-Positive Breast Cancers |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
HCI-13 patient-derived xenograft was treated with vehicle or a tissue-selective androgen receptor modulator (SARM), enobosarm. RNA was extracted from the tumors and microarray was performed.
Objective: The objective of the study is to determine the effect of an androgen receptor (AR) agonist or a selective AR modulator (SARM) on hormone receptor-positive breast cancer growth in preclinical models and to determine the underlying mechanism of action.
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| Overall design |
Methods: Patient-derived xenograft (PDX) HCI-13 (a kind gift from Dr. Alana Whelm, University of Oklahoma) was implanted under the mammary fat pad in female NOD SCID Gamma mice. Once the tumors grew to 100-200 mm3, the animals were randomized and treated orally with vehicle or 10 mg/kg enobosarm. Tumor volume was measured thrice weekly. Thirty days after treatment initiation, the animals were sacrificed and the tumors were snap frozen. RNA was extracted from the tumors using Qiagen RNA isolation kit and its quality, integrity, and quantity were checked. The RNA was then subjected to microarray
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| Contributor(s) |
Narayanan R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 08, 2019 |
| Last update date |
Aug 03, 2019 |
| Contact name |
Daniel Lee Johnson |
| E-mail(s) |
djohn166@uthsc.edu
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| Phone |
9014483743
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| Organization name |
UTHSC
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| Department |
Molecular Informatics Core
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| Street address |
71 S Manassas
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| City |
Memphis |
| State/province |
Tennessee |
| ZIP/Postal code |
38163 |
| Country |
USA |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA521539 |
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