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| Status |
Public on Jul 22, 2020 |
| Title |
mRNA-sequencing for MM.1S cells that were treated with pomalidomide or were expressing shRNA against ARID2 or Aiolos. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To dissect the roles of ARID2 and Aiolos in pomalidomide-induced transcriptional changes, we performed mRNA-sequencing of MM.1S cells expressing shRNA against ARID2 or Aiolos and compared them with MM.1S cells that were treated with pomalidomide for 24, 48, or 72 hours.
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| Overall design |
We performed mRNA-sequencing for MM.1S cells that were treated with 1 µM pomalidomide for 0, 24, 48, or 72 hours or were expressing shRNA against ARID2 or Aiolos for 72 hours (7 conditions with 3 biological replicates). Sequencing was done on the NovaSeq 6000 system (Illumina).
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| Contributor(s) |
Yamamoto J, Yamaguchi Y |
| Citation(s) |
32958952 |
| Submission date |
Feb 12, 2019 |
| Last update date |
Oct 21, 2020 |
| Contact name |
Yuki Yamaguchi |
| E-mail(s) |
yyamaguc@bio.titech.ac.jp
|
| Organization name |
Tokyo Institute of Technology
|
| Department |
School of Life Science and Technology
|
| Street address |
Nagatsutacho 4259
|
| City |
Yokohama |
| ZIP/Postal code |
226-8501 |
| Country |
Japan |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA521983 |
| SRA |
SRP185722 |
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