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| Status |
Public on Dec 31, 2019 |
| Title |
Placental effects on maternal brain revealed by disrupted placental gene expression in mouse hybrids |
| Organisms |
Mus musculus domesticus; Mus spretus; Mus musculus domesticus x Mus spretus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The mammalian placenta is both the physical interface between mother and fetus, and the source of endocrine signals that target the maternal hypothalamus, priming females for parturition, lactation and motherhood. Despite the importance of this connection, the effects of altered placental signaling on the maternal brain are understudied. Here, we show that placental dysfunction alters gene expression in the maternal brain, with the potential to affect maternal behavior. Using a cross between the house mouse and the Algerian mouse in which hybrid placental development is abnormal, we sequenced late gestation placental and maternal medial preoptic area transcriptomes and quantified differential expression and placenta-maternal brain co-expression between normal and hybrid pregnancies. The expression of Fmn1, Drd3, Caln1 and Ctsr was significantly altered in the brains of females exposed to hybrid placentas. Most strikingly, expression patterns of placenta-specific gene families and Drd3 in the brains of house mouse females carrying hybrid litters matched those of female Algerian mice, the paternal species in the cross. Our results indicate that the paternally-derived placental genome can influence the expression of maternal-fetal communication genes, including placental hormones, suggesting an effect of the offspring's father on the mother’s brain.
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| Overall design |
Analysis of 15 RNA samples of the maternal medial preotic area at e17.5 of pregnancy (3 groups, 5 biological replicates each) and 29 samples of placental DNA at e17.5 of the same pregnancies (3 groups, 4-5 biological replicates per sex, per group)
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| Contributor(s) |
Arévalo L, Campbell P |
| Citation(s) |
31937228 |
| Submission date |
Feb 12, 2019 |
| Last update date |
Mar 31, 2020 |
| Contact name |
Polly Campbell |
| E-mail(s) |
polly.campbell@ucr.edu
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| Organization name |
University of California Riverside
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| Department |
Evolution, Ecology, and Organismal Biology
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| Street address |
3401 Watkins Drive
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| City |
Riverside |
| State/province |
CA |
| ZIP/Postal code |
92521 |
| Country |
USA |
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| Platforms (3) |
| GPL26184 |
Illumina HiSeq 4000 (Mus musculus domesticus) |
| GPL26185 |
Illumina HiSeq 4000 (Mus spretus) |
| GPL26187 |
Illumina HiSeq 4000 (Mus musculus domesticus x Mus spretus) |
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| Samples (44)
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| Relations |
| BioProject |
PRJNA521990 |
| SRA |
SRP185733 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE126469_MPA_DOM_raw_counts.csv.gz |
547.3 Kb |
(ftp)(http) |
CSV |
| GSE126469_MPA_DOMvsDOMhl_DESeq2_DE.txt.gz |
1.1 Mb |
(ftp)(http) |
TXT |
| GSE126469_MPA_DOMvsDOMhl_DESeq2_counts.txt.gz |
1.6 Mb |
(ftp)(http) |
TXT |
| GSE126469_MPA_SPRET_raw_counts.csv.gz |
336.1 Kb |
(ftp)(http) |
CSV |
| GSE126469_MPA_SPRETvsDOM_DESeq2_DE.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
| GSE126469_MPA_SPRETvsDOM_DESeq2_counts.txt.gz |
1.7 Mb |
(ftp)(http) |
TXT |
| GSE126469_MPA_SPRETvsDOMhl_DESeq2_DE.txt.gz |
1.4 Mb |
(ftp)(http) |
TXT |
| GSE126469_MPA_SPRETvsDOMhl_DESeq2_counts.txt.gz |
1.7 Mb |
(ftp)(http) |
TXT |
| GSE126469_Placenta_DOMvsSPRET_DESeq2_DE.txt.gz |
1.2 Mb |
(ftp)(http) |
TXT |
| GSE126469_Placenta_DOMvsSPRET_DESeq2_counts.txt.gz |
2.9 Mb |
(ftp)(http) |
TXT |
| GSE126469_Placenta_HYBRIDvsDOM_DESeq2_DE.txt.gz |
1.2 Mb |
(ftp)(http) |
TXT |
| GSE126469_Placenta_HYBRIDvsDOM_DESeq2_counts.txt.gz |
2.8 Mb |
(ftp)(http) |
TXT |
| GSE126469_Placenta_HYBRIDvsSPRET_DESeq2_DE.txt.gz |
1.2 Mb |
(ftp)(http) |
TXT |
| GSE126469_Placenta_HYBRIDvsSPRET_DESeq2_counts.txt.gz |
2.8 Mb |
(ftp)(http) |
TXT |
| GSE126469_Placenta_raw_counts.csv.gz |
1.3 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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