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| Status |
Public on Apr 13, 2021 |
| Title |
Gene expression data from Tyr::CreErT2::BrafV600E Pten F/+ mouse tumors according to defloxing status of Brn2 [tumors] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate the mechanism underlying the effect of Brn2-loss we extracted mRNA from Braf-Pten-Brn2-wt, Braf-Pten-Brn2-het, and Braf-Pten-Brn2-hom mouse melanomas and performed microarray-based transcriptome analysis.
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| Overall design |
We studied the effect of Brn2 loss on in vivo melanomagenesis by generating the mouse lines Tyr::CreERt2/°; BrafV600E/+; Brn2F/+ (Braf-Brn2-het), Tyr::CreERt2/°; BrafV600E/+; Brn2F/F (Braf-Brn2-hom), Tyr::CreERt2/°; BrafV600E/+; PtenF/+; Brn2F/+ (Braf-Pten-Brn2-het) and Tyr::CreERt2/°; BrafV600E/+; PtenF/+; Brn2F/F (Braf-Pten-Brn2-hom) by introducing the floxed Brn2 locus into the genome by appropriate crossings. Cre-mediated defloxing of Braf, Pten, and Brn2 loci was induced by topical application of tamoxifen during the first three days after birth. All mice were monitored for the number of tumors/mice, and the growth rate of the first tumor.
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| Contributor(s) |
Hamm M, Sohier P, Raymond JH, Larue L |
| Citation(s) |
34140478 |
| Submission date |
Feb 13, 2019 |
| Last update date |
Jul 08, 2021 |
| Contact name |
Lionel Larue |
| Phone |
+33 1 69 86 71 07
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| Organization name |
Institut Curie
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| Department |
UMR3347 CNRS - U1021 INSERM, Institut Curie
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| Lab |
Normal and Pathological Development of Melanocytes
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| Street address |
Bat 110, Centre universitaire
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| City |
Orsay |
| ZIP/Postal code |
91405 |
| Country |
France |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (25)
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| This SubSeries is part of SuperSeries: |
| GSE163086 |
Gene expression data from Tyr::CreErT2::BrafV600E Pten F/+ mouse tumors according to defloxing status of Brn2 |
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| Relations |
| BioProject |
PRJNA522269 |
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