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| Status |
Public on Feb 15, 2019 |
| Title |
Affymetrix GeneChip miRNA 4.0 Array for meningioma recurrence profilling |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Variability of meningioma growth, even within benign subgroup, makes some remain unaltered, while others grow fast despite an appended radiotherapy. Biomarkers that differentiate between less and more aggressive meningiomas would therefore have a significant clinical benefit in prediction of their biological behaviour. The aim of the study was to identify new candidate miRNAs enabling better and proper prediction of meningioma recurrence. We used miRNA 4.0 array (Applied Biosystems, Foster City, CA, USA) to detect miRNA profiles in 44 primary meningioma patients (19 with subsequent recurrence) and 5 healthy controls. Moreover, miRNA profiles were analyzed in 15 secondary meningiomas.
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| Overall design |
Affymetrix GeneChip miRNA 4.0 Arrays were performed according to the manufacturer's directions on total RNA extracted from macrodisected FFPE meningioma samples.
MiRNA expression analysis of Affymetrix GeneChip miRNA 4.0 Arrays was performed at 45 meningioma patients and 5 healthy controls. Healthy control samples were not included in the final analysis.
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| Contributor(s) |
Srovnal J, Balik V |
| Citation missing |
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| Submission date |
Feb 14, 2019 |
| Last update date |
Feb 16, 2019 |
| Contact name |
Josef Srovnal |
| E-mail(s) |
josef.srovnal@upol.cz
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| Organization name |
Palacky University
|
| Department |
IMTM
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| Street address |
Hnevotinska 5
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| City |
Olomouc |
| ZIP/Postal code |
77900 |
| Country |
Czech Republic |
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| Platforms (1) |
| GPL19117 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array |
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| Samples (64)
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| Relations |
| BioProject |
PRJNA522376 |
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