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| Status |
Public on Jan 05, 2020 |
| Title |
Genome-wide maps of Qki-5 and PPARb in mouse oligodendrocytes |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We found that there is a big overlap between the Qki5- and PPARb-binding events by ChIP-seq. Interestingly, the genomic distribution analyses of Qki-5- and PPARĪ²-binding events showed significant enrichment at the regions of the promoter/transcription start site (TSS) which were indicated by Pol II-binding events. The strong preference of the promoter/TSS co-occupancy suggested that Qki-5 might play an important role in PPARĪ²-mediated transcriptional regulation of its downstream genes.
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| Overall design |
Examination of genome-wide maps Qki-5 and PPARb in oligodendrocytes which are differentiated from neural stem cells or oligodendrocytes freshly isolated from mouse brains by ChIP-seq. Neural stem cells were differentiated in Neurobasal medium containing B-27, 2 mM GlutaMAX-I, 100 ng/mL insulin-like growth factor 1 (IGF-1), and 50 ng/mL 3,3',5-Triiodo-L-thyronine (T3). Oligodendrocytes were freshly isolated from mouse brains using O4 magnetic beads.
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| Contributor(s) |
He C, Zhou X, Lan F, Hu J |
| Citation(s) |
32202512 |
| Submission date |
Feb 14, 2019 |
| Last update date |
Apr 05, 2020 |
| Contact name |
Chenxi He |
| E-mail(s) |
cxhe15@fudan.edu.cn
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| Organization name |
Fudan University
|
| Street address |
131 Dong'an Rd, Mingdao Bldg, Rm 512
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
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| Platforms (2) |
| GPL21273 |
HiSeq X Ten (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA522428 |
| SRA |
SRP185910 |
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