|
| Status |
Public on May 01, 2019 |
| Title |
DNA methylation analysis of NNMT overexpression and knockdown in stromal cells |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
|
| Summary |
Genome-wide DNA methylation profiling in response to overexpression or knockdown of NNMT in stromal cells. The Illumina MethylationEPIC array was used to profiled genome-wide methylation of normal fibroblasts expressing a control construct or NNMT overexpression construt and cancer-associated fibroblasts (CAFs) expressing an shCtrl or shNNMT construct. Two biological replicates of each experimental group were profiled following growth in 10 micromolar methionine growth media for 21 days.
|
| |
|
| Overall design |
Bisulphite converted DNA from the 8 samples (4 experimental groups with two biological replicates per group) were hybridised to the Illumina InfiniumMethylationEPIC array.
|
| |
|
| Contributor(s) |
Eckert MA, Lengyel E |
| Citation(s) |
31043742 |
| Submission date |
Feb 17, 2019 |
| Last update date |
May 31, 2019 |
| Contact name |
Mark Adam Eckert |
| E-mail(s) |
meckert@bsd.uchicago.edu
|
| Organization name |
University of Chicago
|
| Department |
OB/GYN
|
| Lab |
Lengyel Lab
|
| Street address |
929 East 57th St, W107
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (8)
|
| GSM3611046 |
200932680194_R01C01: WI-38 fibroblasts Ctrl Replicate 1 |
| GSM3611047 |
200932680194_R02C01: WI-38 fibroblasts Ctrl Replicate 2 |
| GSM3611048 |
200932680194_R03C01: WI-38 fibroblasts NNMT Replicate 1 |
| GSM3611049 |
200932680194_R04C01: WI-38 fibroblasts NNMT Replicate 2 |
| GSM3611050 |
200932680194_R05C01: CAFs shCtrl Replicate 1 |
| GSM3611051 |
200932680194_R06C01: CAFs shCtrl Replicate 2 |
| GSM3611052 |
200932680194_R07C01: CAFs shNNMT Replicate 1 |
| GSM3611053 |
200932680194_R08C01: CAFs shNNMT Replicate 2 |
|
| Relations |
| BioProject |
PRJNA522911 |
Less...
More...