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| Status |
Public on May 10, 2019 |
| Title |
Transcriptome analysis of activated fibroblasts after intratracheal transfer in bleomycin-induced lung fibrosis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Intratracheal transfer of isolated lung fibroblasts in bleomycin-induced lung fibrosis recapitulates the activation process of lung fibroblasts after epithelial injury. In order to investigate gene expression signatures of transferred fibroblasts, we purified transferred fibroblasts 2, 4, and 7 days after the transfer and performed transcriptome analysis. We also isolated Acta2 high and low cells by using Acta2-mKO1 reporter mice 4 days after the transfer.
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| Overall design |
Lung fibroblasts were isolated from untreated Col-GFP mice after tissue dissociation and negative selection for lineage markers. Isolated lung fibroblasts were intratracheally transferred into wild type mice, which received intratracheal bleomycin treatment 7 days before the transfer. Col-GFP+ cells were purified from the host lungs by FACS sorting on 2, 4, and 7 days after the transfer. Acta2 high and low cells were prepared by transferring lung fibroblasts from Acta2-mKO1 reporter mice. mRNA was isolated from sorted cells, and gene expression profiles were acquired by next generation sequencing.
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| Contributor(s) |
Tsukui T, Hashimoto S, Shichino S |
| Citation(s) |
31078262 |
| Submission date |
Feb 18, 2019 |
| Last update date |
May 14, 2019 |
| Contact name |
Tatsuya Tsukui |
| E-mail(s) |
tatsuya.tsukui@ucsf.edu
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| Organization name |
UCSF
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| Department |
Medicine
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| Street address |
555 Mission Bay Blvd South, 282
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| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA523014 |
| SRA |
SRP186182 |
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