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| Status |
Public on Feb 02, 2020 |
| Title |
Diffuse glioma cells with perineural satellitosis invade by mediating metabolic reprogramming |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We developed diffuse glioma cells with H3F3A_K27M mutation.K27M diffuse glioma cells have high migration ability and maintain close contact with neurons with immature neuron-like properties.K27M cells produce neurotrophic factors and lactic acid and affect neurite growth and differentiation/suppression of surrounding neurons. K27M diffuse glioma cells show an invasion type and gene expression patterns similar to those of human glioma.
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| Overall design |
We prepared immortalized murine glial cells (OS3; established and obtained from C3H male mouse brain) with H3.3-K27M mutation and control glial cells with the empty vector. We harvested three dishes in each cohort (n=3) and extracted RNA from each dishes. Finally, we perfomred RNA microarray analysis.
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| Contributor(s) |
Tomita H, Miyai M |
| Citation(s) |
33506198, 36353180 |
| Submission date |
Feb 19, 2019 |
| Last update date |
Nov 29, 2022 |
| Contact name |
Hiroyuki Tomita |
| Organization name |
Gifu University
|
| Street address |
1-1 Yanagido
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| City |
Gifu |
| ZIP/Postal code |
5011194 |
| Country |
Japan |
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| Platforms (1) |
| GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA523259 |
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